Enzymes having alpha-galactosidase activity and methods of use thereof

Abstract

The invention relates to α-galactosidase and to polynucleotides encoding the α-galactosidase. In addition methods of designing new α-galactosidases and method of use thereof are also provided. The α-galactosidases have increased activity and stability at increased pH and temperature.

What is claimed is:  
     
         1 . An isolated nucleic acid comprising a sequence A purified antibody that specifically binds to a polypeptide comprising a sequence as set forth in SEQ ID NO: 4, and sequences substantially identical thereto.  
     
     
         2 . A purified antibody that specifically binds to a polypeptide having at least 10 consecutive amino acids of the polypeptides as set forth in SEQ ID NO: 4, and sequences substantially identical thereto.  
     
     
         3 . The antibody of  claim 1  or  2 , wherein the antibodies are polyclonal.  
     
     
         4 . The antibody of  claim 1  or  2 , wherein the antibodies are monoclonal.