Enzymes having alpha-galactosidase activity and methods of use thereof

Abstract

The invention relates to α-galactosidase and to polynucleotides encoding the α-galactosidase. In addition methods of designing new α-galactosidases and method of use thereof are also provided. The α-galactosidases have increased activity and stability at increased pH and temperature.

What is claimed is:  
     
         1 . A computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a nucleic acid sequence as set forth in SEQ ID NO: 3, and sequences substantially identical thereto, or a polypeptide sequence as set forth in SEQ ID NO: 4, and sequences substantially identical thereto.  
     
     
         2 . The computer system of  claim 1 , further comprising a sequence comparison algorithm and a data storage device having at least one reference sequence stored thereon.  
     
     
         3 . The computer system of  claim 2 , wherein the sequence comparison algorithm comprises a computer program which indicates polymorphisms.  
     
     
         4 . The computer system of  claim 1 , further comprising an identifier which identifies features in said sequence.