Methods and compositions relating to bone-derived growth factor-like polypeptides

Abstract

The present invention provides novel polynucleotides and proteins encoded by such polynucleotides, along with therapeutic, diagnostic and research utilities for these polynucleotides and proteins. In particular, the polypeptides of the invention comprise amino acid sequences with similarity to bone-derived growth factor and quiescin sequences.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising: 
 a) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2;    b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence encoded by the cDNA insert of clone pBDGF-HY1 deposited under accession number ATCC ______;    c) a nucleotide sequence comprising the full length protein coding sequence of a cDNA comprising the cDNA insert of clone pBDGF-HY1 deposited under accession number ATCC ______;    d) a nucleotide sequence encoding the mature protein encoded by the cDNA insert of clone pBDGF-HY1 deposited under accession number ATCC ______.    e) the nucleotide sequence of SEQ ID NO:1;    f) nucleotides 1-669 of the nucleotide sequence of SEQ ID NO:1;    g) a nucleotide sequence of the cDNA insert of clone PBDGF-HY1 deposited under accession number ATCC ______; or    h) a nucleotide sequence of the mature protein coding sequence of clone pBDGF-HY1 deposited under accession number ATCC ______.    
     
     
         2 . An isolated polynucleotide which hybridizes to the complement of the polynucleotide of  claim 1  under stringent hybridization conditions.  
     
     
         3 . An isolated polynucleotide which comprises the complement of the polynucleotide of  claim 1 .  
     
     
         4 . A vector comprising the isolated polynucleotide of  claim 1  or  2 .  
     
     
         5 . An expression vector comprising the isolated polynucleotide of  claim 1  or  2 .  
     
     
         6 . A host cell genetically engineered to contain the polynucleotide of  claim 1  or  2 .  
     
     
         7 . A host cell genetically engineered to contain the polynucleotide of  claim 1  or  2  in operative association with a regulatory sequence that controls expression of the polynucleotide in the host cell.  
     
     
         8 . An isolated polypeptide comprising: 
 a) the amino acid sequence of SEQ ID NO:2;    b) the amino acid sequence encoded by-the cDNA insert of clone pBDGF-HY1 deposited under accession number ATCC ______;    c) an amino acid sequence comprising the full length protein encoded by a cDNA comprising the cDNA insert of clone PBDGF-HY1 deposited under accession number ATCC ______;    d) a polypeptide comprising the mature protein encoded by a cDNA comprising the cDNA insert of clone pBDGF-HY1 deposited under accession number ATCC ______; or    e) a mature protein encoded by the cDNA insert of clone pBDGF-HY1 deposited under accession number ATCC ______.    
     
     
         9 . A composition comprising the polypeptide of  claim 8  and a carrier.  
     
     
         10 . An antibody directed against the polypeptide of  claim 8 .  
     
     
         11 . A method for detecting a polynucleotide of  claim 1  or  2  in a sample, comprising: 
 a) contacting the sample with a compound that binds to and forms a complex with the polynucleotide for a period sufficient to form the complex; and  
 b) detecting the complex,  
 so that if a complex is detected, a polynucleotide of  claim 1  or  2  is detected.  
 
     
     
         12 . A method for detecting a polynucleotide of  claim 1  or 2 in a sample, comprising: 
 a) contacting the sample under stringent hybridization conditions with nucleic acid primers that anneal to a polynucleotide of  claim 1  or  2  under such conditions; and  
 b) amplifying the annealed polynucleotides,  
 so that if a polynucleotide is amplified, a polynucleotide of  claim 1  or  2  is detected.  
 
     
     
         13 . The method of  claim 12 , wherein the polynucleotide is an RNA molecule that encodes a polypeptide of  claim 8 , and the method further comprises reverse transcribing an annealed RNA molecule into a cDNA polynucleotide.  
     
     
         14 . A method for detecting a polypeptide of  claim 8  in a sample, comprising: 
 a) contacting the sample with a compound that binds to and forms a complex with the polypeptide for a period sufficient to form the complex; and  
 b) detecting the complex,  
 so that if a complex is detected, a polypeptide of  claim 8  is detected.  
 
     
     
         15 . A method for identifying a compound that binds to a polypeptide of  claim 8 , comprising: 
 a) contacting a compound with a polypeptide of  claim 8  for a time sufficient to form a polypeptide/compound complex; and    b) detecting the complex,    so that if a polypeptide/compound complex is detected, a compound that binds to a polypeptide of  claim 8  is identified.    
     
     
         16 . A method for identifying a compound that binds to a polypeptide of  claim 8 , comprising: 
 a) contacting a compound with a polypeptide of  claim 8 , in a cell, for a time sufficient to form a polypeptide/compound complex, wherein the complex drives expression of a reporter gene sequence in the cell; and    b) detecting the complex by detecting reporter gene sequence expression,    so that if a polypeptide/compound complex is detected, a compound that binds to a polypeptide of  claim 8  is identified.    
     
     
         17 . A method of modulating activity of a polypeptide of  claim 8 , comprising contacting a cell that expresses the polypeptide with a compound that modulates activity of the polypeptide for a time sufficient to modulate said activity.  
     
     
         18 . A method of modulating activity of the polypeptide of  claim 8 , comprising contacting the polypeptide with a compound that modulates activity of the polypeptide for a time sufficient to modulate said activity.