DNA polymerase-related factors

Abstract

The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.

1 . A method of DNA synthesis by using a DNA polymerase, comprising synthesizing DNA in the presence of a DNA polymerase-associated factor possessing an activity of enhancing DNA synthesizing-activity of a DNA polymerase, wherein the factor comprises at least one of amino acid sequences selected from the group consisting of SEQ ID NOS: 1, 3, 19, 27, 34, 64, 70 and 80.  
     
     
         2 . The method of DNA synthesis according to  claim 1 , wherein DNA is synthesized in the presence of two or more kinds of isolated DNA polymerase-associated factors.  
     
     
         3 . The method of DNA synthesis according to  claim 2 , wherein DNA is synthesized in the presence of F7, PFU-RFC and PFU-RFCLS as an isolated DNA polymerase-associated factor.  
     
     
         4 . The method of DNA synthesis according to any one of claims  1 - 3 , wherein said DNA polymerase is a thermostable DNA polymerase.  
     
     
         5 . The method of DNA synthesis according to  claim 4 , wherein the synthesis is carried out by PCR method.