Extending tissue preservation

Abstract

A method of preserving tissue by placing the tissue in a media containing pure perfluorocarbon is provided. Also provided is a tissue preserving media including storage media and pure perfluorocarbon. A method of making media by adding pure perfluorocarbon to the media is provided. A method of preserving tissue by oxygenating the storage media by adding pure perfluorocarbons to the media is also provided.

What is claimed is:  
     
         1 . A method of preserving a tissue or cell by placing the tissue or cell in a media including a perfluorocarbon.  
     
     
         2 . The method of  claim 1  further including emulsifying the perfluorocarbon.  
     
     
         3 . The method of  claim 1  further including oxygenating the perfluorocarbon.  
     
     
         4 . A tissue media comprising a storage media and a pure perfluorocarbon.  
     
     
         5 . The media of  claim 4  in which said storage media is selected from the group consisting essentially of Optisol, Dexsol, McCarey-Kaufman medium, K-Sol, Corneal Storage Medium (CSM), Minimal Essential Medium (MEM), H-Sol, Physiological Salne Solution, Eagle's Media, cell culture media, tissue culture media, cell storage media, and tissue storage media.  
     
     
         6 . The media of  claim 4  in which said pure perfluorocarbon is at least 90% pure perfluorocarbon.  
     
     
         7 . The media of  claim 6  in which said pure perfluorocarbon is at least 95% pure perfluorocarbon.  
     
     
         8 . The media of  claim 6  in which said pure perfluorocarbon is an emulsion.  
     
     
         9 . The media of  claim 8  in which said emulsion particle size is ≦0.5 micron.  
     
     
         10 . The media of  claim 4  in which said pure perfluorocarbon structure is cyclical.  
     
     
         11 . The media of  claim 10  in which said pure perfluorocarbon is selected from the group consisting essentially of perfluorodecalin, perfluorotripropylamine, perfluoro-N-methyl-decahydroisoquinoline, perfluoromethylcyclohexylpiperidine, perfluorodimethylcyclonanes and perfluoromethyladamantane.  
     
     
         12 . The media of  claim 4  in which said pure perfluorocarbon structure is a linear polymer.  
     
     
         13 . The media of  claim 12  in which said pure perfluorocarbon is selected from the group consisting essentially of perfluorooctylbromide, bis(perfluoro-butyl)ethane perfluoro-octane and perfluoro-octane.  
     
     
         14 . The media of  claim 4  in which said pure perfluorocarbon is oxygenated.  
     
     
         15 . The media of  claim 14  in which said pure perfluorocarbon is oxygenated with an oxygen and carbon dioxide mixture.  
     
     
         16 . The media of  claim 15  in which said oxygen and said carbon dioxide mixture is a 95:5% mixture.  
     
     
         17 . The media of  claim 4  in which a volume ratio of pure perfluorocarbon is in the range of 1 to 95%.  
     
     
         18 . The media of  claim 17  in which said pure perfluorocarbon volume ratio range is 5 to 30%.  
     
     
         19 . The media of  claim 18  in which said pure perfluorocarbon volume ratio is 10%.  
     
     
         20 . The media of  claim 4  in which said media further contains an antifreeze protein.  
     
     
         21 . The media of  claim 20  in which said media further contains one or more compounds selected from the group consisting essentially of citrate, succinate, malate, aspartate, glutamate, ammonium sulfate, glycerol, sorbitol, alanine and ammonium bicarbonate.  
     
     
         22 . A method of making a tissue storage media that is oxygenated by adding a perfluorocarbon.  
     
     
         23 . A method of preserving a tissue by placing the tissue in an oxygenated storage media containing a perfluorocarbon.