Polypeptide, cdna encoding the polypeptide, and use of the both

Abstract

A new polypeptide prepared from human library by the SST method and a process for preparation of it; a cDNA encoding the polypeptide; a fragment selectively hybridizing with the sequence of the cDNA; a replication or expression plasmid containing the cDNA integrated thereinto; a host cell transformed with plasmid; an antibody against the polypeptide; and a pharmaceutical composition containing the polypeptide or the antibody.

1 . A substantially purified form of the polypeptide comprising the amino acid sequence shown in SEQ ID NOS. 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76 or 79, homologue thereof, fragment thereof or homologue of the fragment.  
     
     
         2 . A polypeptide according to  claim 1  comprising the amino acid sequence shown in SEQ ID NOS. 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76 or 79.  
     
     
         3 . A cDNA encoding the polypeptide according to  claim 1 .  
     
     
         4 . A cDNA according to  claim 3  comprising the nucleotide sequence shown in SEQ ID NOS. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77 or 80, or a fragment cDNA selectively hybridized to the cDNA.  
     
     
         5 . A cDNA according to  claim 3  comprising the nucleotide sequence shown in SEQ ID NOS. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78 or 81, or a fragment cDNA selectively hybridized to the cDNA.  
     
     
         6 . A replication or expression vector carrying the cDNA according to  claims 3  to  5 .  
     
     
         7 . A host cell transformed with the replication or expression vector according to  claim 6 .  
     
     
         8 . A method for producing the polypeptide according to  claim 1  or  2  which comprises culturing a host cell according to  claim 7  under a condition effective to express the polypeptide according to  claim 1  or  2 .  
     
     
         9 . A monoclonal or polyclonal antibody against the polypeptide according to  claim 1  or  2 .  
     
     
         10 . A pharmaceutical composition containing the polypeptide according to  claim 1  or  2  or the antibody according to  claim 9 , in association with pharmaceutically acceptable diluent and/or carrier.