Leucine-rich repeat proteins, Zlrr7, Zlrr8 and Zlrr9

Abstract

The present invention provides to polynucleotides and secreted Leucine-Rich Repeat proteins encoded by the polynucleotides. The proteins include binding proteins and fusion proteins operably linked to a second polypeptide. The invention further provides therapeutic and diagnostic methods utilizing the polynucleotides, polypeptides, and antagonists of the polypeptides.

We claim:  
     
         1 . An isolated polypeptide comprising residues 27 to 234 as shown in SEQ ID NO:2.  
     
     
         2 . The isolated polypeptide of  claim 1  wherein the polypeptide further comprises residues 1 to 234 as shown in SEQ ID NO:2.  
     
     
         3 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 1 .  
     
     
         4 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of  claim 3;  and    a transcription terminator.    
     
     
         5 . A cultured cell comprising the expression vector of  claim 4 .  
     
     
         6 . A method of producing a polypeptide comprising culturing the cell of  claim 5  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         7 . A polypeptide produced by the method of  claim 6 .  
     
     
         8 . An antibody that specifically binds to the isolated protein of  claim 1 .  
     
     
         9 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO: 1.  
     
     
         10 . An isolated polypeptide comprising residues 16 to 279 as shown in SEQ ID NO:8.  
     
     
         11 . The isolated polypeptide of  claim 10  wherein the polypeptide further comprises residues 16 to 487 as shown in SEQ ID NO:8.  
     
     
         12 . The isolated polypeptide of  claim 10  wherein the polypeptide further comprises residues 1 to 487 as shown in SEQ ID NO:8.  
     
     
         13 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 10 .  
     
     
         14 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  13 ; and    a transcription terminator.    
     
     
         15 . A cultured cell comprising the expression vector of  claim 14 .  
     
     
         16 . A method of producing a polypeptide comprising culturing the cell of  claim 15  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         17 . A polypeptide produced by the method of  claim 16 .  
     
     
         18 . An antibody that specifically binds to the isolated protein of  claim 10 .  
     
     
         19 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:7.  
     
     
         20 . An isolated polypeptide comprising residues 16 to 144 as shown in SEQ ID NO:5.  
     
     
         21 . The isolated polypeptide of  claim 20  wherein the polypeptide further comprises residues 1 to 144 as shown in SEQ ID NO:8.  
     
     
         22 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 20 .  
     
     
         23 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  22 ; and    a transcription terminator.    
     
     
         24 . A cultured cell comprising the expression vector of  claim 23 .  
     
     
         25 . A method of producing a polypeptide comprising culturing the cell of  claim 24  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         26 . A polypeptide produced by the method of  claim 25 .  
     
     
         27 . An antibody that specifically binds to the isolated protein of  claim 20 .  
     
     
         28 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:4.  
     
     
         29 . An isolated polypeptide comprising residues 76 to 363 as shown in SEQ ID NO: 14.  
     
     
         30 . The isolated polypeptide of  claim 29  wherein the polypeptide further comprises residues 76 to 663 as shown in SEQ ID NO: 14.  
     
     
         31 . The isolated polypeptide of  claim 29  wherein the polypeptide further comprises residues 1 to 663 as shown in SEQ ID NO: 14.  
     
     
         32 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 29 .  
     
     
         33 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  32 ; and    a transcription terminator.    
     
     
         34 . A cultured cell comprising the expression vector of  claim 33 .  
     
     
         35 . A method of producing a polypeptide comprising culturing the cell of  claim 34  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         36 . A polypeptide produced by the method of  claim 35 .  
     
     
         37 . An antibody that specifically binds to the isolated protein of  claim 29 .  
     
     
         38 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:13.  
     
     
         39 . An isolated polypeptide comprising residues 76 to 278 as shown in SEQ ID NO: 11.  
     
     
         40 . The isolated polypeptide of  claim 39  wherein the polypeptide further comprises residues 1 to 278 as shown in SEQ ID NO: 11.  
     
     
         41 . An isolated polynucleotide comprising a sequence of nucleotides, wherein the sequence encodes the isolated polypeptide of  claim 39 .  
     
     
         42 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment having the isolated polynucleotide of claim  39 ; and    a transcription terminator.    
     
     
         43 . A cultured cell comprising the expression vector of  claim 42 .  
     
     
         44 . A method of producing a polypeptide comprising culturing the cell of  claim 43  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         45 . A polypeptide produced by the method of  claim 44 .  
     
     
         46 . An antibody that specifically binds to the isolated protein of  claim 39 .  
     
     
         47 . An isolated polynucleotide comprising the polynucleotide sequence as shown in SEQ ID NO:10.