Disintegrin homologs

Abstract

The present invention relates to polynucleotide and polypeptide molecules for zdint1, a novel member of the Disintegrin Proteases. The polypeptides, and polynucleotides encoding them, are believed to be cell-cell interaction modulating and may be used for delivery and therapeutics. The present invention also includes antibodies to the zdint1 polypeptides.

What is claimed is:  
     
         1 . An isolated polypeptide molecule comprising a contiguous sequence of 14 amino acids of SEQ ID NO:2.  
     
     
         2 . An isolated polypeptide molecule according to  claim 1 , wherein the polypeptide molecule comprises residues 437 to 450 of SEQ ID NO:2.  
     
     
         3 . An isolated polypeptide molecule according to  claim 1  wherein the polypeptide molecule is between 82 and 232 amino acids in length.  
     
     
         4 . An isolated polypeptide molecule according to  claim 3  wherein the polypeptide molecule is residues 164 to 382 of SEQ ID NO:2.  
     
     
         5 . An isolated polypeptide molecule according to  claim 3  wherein the polypeptide molecule is residues 383 to 464 of SEQ ID NO:2.  
     
     
         6 . An isolated polypeptide molecule according to  claim 3  wherein the polypeptide molecule is residues 465 to 696 of SEQ ID NO:2.  
     
     
         7 . A isolated polypeptide molecule selected from the group consisting of: 
 a) a polypeptide molecule comprising residues 164 to 382 of SEQ ID NO:2;    b) a polypeptide molecule comprising residues 383 to 464 of SEQ ID NO:2;    c) a polypeptide molecule comprising residues 465 to 696 of SEQ ID NO:2;    d) a polypeptide molecule comprising residues 438 to 449 of SEQ ID NO:2;    e) a polypeptide molecule comprising residues 164 to 464 of SEQ ID NO:2;    f) a polypeptide molecule comprising residues 164 to 696 of SEQ ID NO:2;    g) a polypeptide molecule comprising residues 383 to 696 of SEQ ID NO:2;    h) a polypeptide molecule comprising residues 164 to 449 of SEQ ID NO:2;    i) a polypeptide molecule comprising residues 438 to 696 of SEQ ID NO:2; and    j) a polypeptide molecule comprising residues 1 to 696 of SEQ ID NO:2.    
     
     
         8 . An isolated polynucleotide molecule encoding a polypeptide molecule, wherein the polypeptide molecule comprises a contiguous sequence of 14 amino acids of SEQ ID NO:2.  
     
     
         9 . An isolated polynucleotide molecule according to  claim 8 , wherein the polypeptide molecule comprises residues 437 to 450 of SEQ ID NO:2.  
     
     
         10 . An isolated nucleotide molecule according to  claim 8 , wherein the polypeptide molecule is between 82 and 232 amino acids in length.  
     
     
         11 . An isolated polynucleotide molecule according to  claim 10 , wherein the polypeptide molecule is residues 164 to 382 of SEQ ID NO:2.  
     
     
         12 . An isolated polynucleotide molecule according to  claim 10 , wherein the polypeptide molecule is residues 383 to 464 of SEQ ID NO:2.  
     
     
         13 . An isolated polynucleotide molecule according to  claim 10 , wherein the polypeptide molecule is residues 465 to 696 of SEQ ID NO:2.  
     
     
         14 . A isolated polynucleotide molecule encoding a polypeptide molecule, wherein the polypeptide molecule is selected from the group consisting of: 
 a) a polypeptide molecule comprising residues 164 to 382 of SEQ ID NO:2;    b) a polypeptide molecule comprising residues 383 to 464 of SEQ ID NO:2;    c) a polypeptide molecule comprising residues 465 to 696 of SEQ ID NO:2;    d) a polypeptide molecule comprising residues 438 to 449 of SEQ ID NO:2;    e) a polypeptide molecule comprising residues 164 to 464 of SEQ ID NO:2;    f) a polypeptide molecule comprising residues 164 to 696 of SEQ ID NO:2;    g) a polypep tide molecule comprising residues 383 to 696 of SEQ ID NO:2;    h) a polypeptide molecule comprising residues 164 to 449 of SEQ ID NO:2;    i) a polypeptide molecule comprising residues 438 to 696 of SEQ ID NO:2; and    j) a polypeptide molecule comprising residues 1 to 696 of SEQ ID NO:2.    
     
     
         15 . An isolated polynucleotide encoding a fusion protein having a first segment and a second segment, wherein the first segment comprises a first polypeptide encoding a polypeptide having a protease domain and the second segment comprises a second polynucleotide encoding a polypeptide that has a contiguous sequence of 14 amino acids between residues 383 and 464 of SEQ ID NO:2, and wherein the first segment is positioned amino-terminally to the second segment.  
     
     
         16 . An isolated polynucleotide according to  claim 15 , wherein the protease domain is selected from the group consisting of; 
 a) a protease domain that is a member of the Disintegrin Proteases; and    b) a protease domain that is at least 80% identical to amino acid residues 164 to 382 of SEQ ID NO:2.    
     
     
         17 . An isolated polynucleotide molecule encoding a polypeptide molecule wherein the polynucleotide molecule is selected from the group consisting of: 
 a) a polynucleotide molecule that encodes a polypeptide molecule that is at least 80% identical to residues 383 to 464 of SEQ ID NO:2; and    b) a polynucleotide molecule that is complementary to a).    
     
     
         18 . An isolated polynucleotide molecule according to  claim 17  wherein the polynucleotide molecule is selected from the group consisting of: 
 a) a polynucleotide molecule that encodes a polypeptide molecule that is at least 80% identical to residues 383 to 696 of SEQ ID NO:2; and  
 b) a polynucleotide -molecule that is complementary to a).  
 
     
     
         19 . An isolated polynucleotide molecule according to  claim 17 , wherein the polynucleotide molecule is selected from the group consisting of: 
 a) a polynucleotide molecule that encodes a polypeptide molecule that is at least 80% identical to residues 1 to 696 of SEQ ID NO:2; and    b) a polynucleotide molecule that is complementary to a).    
     
     
         20 . An expression vector comprising the following operably linked elements: 
 a) a transcription promoter;    b) a DNA segment encoding the polypeptide of  claim 1;  and    c) a transcription terminator.    
     
     
         21 . An expression vector of  claim 20  wherein the DNA segment further encodes an affinity tag.  
     
     
         22 . A cultured cell into which has been introduced an expression vector according to  claim 21 , wherein said cell expresses the polypeptide encoded by the DNA segment.  
     
     
         23 . A method of producing a polypeptide comprising culturing a cell according to  claim 22 , whereby said cell expresses the polypeptide encoded by the DNA segment; and recovering the polypeptide.  
     
     
         24 . A method for modulating cell-cell interactions by combining the polypeptide according to  claim 1 , with cells in vivo and in vitro.  
     
     
         25 . A method for modulating cell-cell interactions according to  claim 24 , whereby the cells are derived from tissues selected from the group consisting of: 
 a) tissues from heart;    b) tissues from brain;    c) tissues from spinal cord; and    d) tissues from skeletal muscle.    
     
     
         26 . An isolated polypeptide molecule comprising a contiguous sequence of amino acids, wherein the contiguous sequence of amino acids is selected from the group consisting of: 
 a) SEQ ID NO:7;    b) SEQ ID NO:8;    c) SEQ ID NO:9;    d) SEQ ID NO:10; and    e) SEQ ID NO:11.    
     
     
         27 . An isolated polynucleotide molecule encoding the isolated polypeptide molecule of claim  26 .