Human regulatory molecules

Abstract

The invention provides human regulatory molecules and the polynucleotides which identify and encode them. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention further provides methods for diagnosing and treating disorders associated with expression of human regulatory molecules.

What is claimed is:  
     
         1 . A purified protein comprising an amino acid sequence selected from SEQ ID NOs: 1-49.  
     
     
         2 . An isolated polynucleotide comprising a nucleic acid sequence encoding the protein of  claim 1  or the complement of the polynucleotide.  
     
     
         3 . A composition comprising a polynucleotide of  claim 2  and a reporter molecule.  
     
     
         4 . An isolated polynucleotide comprising a nucleic acid sequence selected from SEQ ID NOs:50-98 and the complement of the polynucleotide.  
     
     
         5 . A vector containing the polynucleotide of  claim 2 .  
     
     
         6 . A host cell containing the vector of  claim 5 .  
     
     
         7 . A method for using a polynucleotide to produce a protein comprising: 
 a) culturing the host cell of  claim 6  under conditions for the expression of the protein, and    b) recovering the protein from the host cell culture.    
     
     
         8 . A method for using a polynucleotide to detect expression of a nucleic acid in a sample, the method comprising: 
 a) hybridizing the polynucleotide of  claim 2  to nucleic acids of the sample, thereby forming a hybridization complex, and    b) detecting hybridization complex formation, wherein complex formation indicates the expression of the polynucleotide in the sample.    
     
     
         9 . The method of  claim 8  wherein the polynucleotide is attached to a substrate or bonded to the surface of a microarray.  
     
     
         10 . The method of  claim 8  wherein the nucleic acids of the sample are amplified prior to hybridization.  
     
     
         11 . A method of using a polynucleotide to screen a plurality of molecules to identify a ligand, the method comprising: 
 a) combining the polynucleotide of  claim 2  with a plurality of molecules under conditions to allow specific binding, and    b) detecting specific binding, thereby identifying a ligand which specifically binds the polynucleotide.    
     
     
         12 . The method of  claim 11  wherein the molecules are selected from DNA molecules, RNA molecules, peptide nucleic acids, artificial chromosome constructions, peptides, and transcription factors.  
     
     
         13 . A method for diagnosing a disease associated with gene expression in a sample containing nucleic acids, the method comprising: 
 a) hybridizing a polynucleotide of  claim 2  to nucleic acids of the sample under conditions to form a hybridization complex,    b) comparing hybridization complex formation with standards, thereby diagnosing the disease.    
     
     
         14 . The method of  claim 13  wherein expression is diagnostic of cancer or immune response.  
     
     
         15 . A composition comprising the protein of  claim 1  and a pharmaceutical carrier or a labeling moiety.  
     
     
         16 . A method for using a protein to screen a plurality of molecules to identify a ligand, the method comprising: 
 a) combining the protein of  claim 1  with the molecules under conditions to allow specific binding, and    b) detecting specific binding, thereby identifying a ligand which specifically binds the protein.    
     
     
         17 . The method of  claim 16  wherein the molecules are selected from DNA molecules, RNA molecules, peptide nucleic acids, peptides, pharmaceutical agents, proteins, mimetics, agonists, antagonists, Ned antibodies, immunoglobulins, inhibitors, and drugs.  
     
     
         18 . A method of using a protein to prepare and purify antibodies comprising: 
 a) immunizing a animal with the protein of  claim 1  under conditions to elicit an antibody response,    b) isolating animal antibodies,    c) attaching the protein to a substrate,    d) contacting the substrate with isolated antibodies under conditions to allow specific binding to the protein,    e) dissociating the antibodies from the protein, thereby obtaining purified antibodies.    
     
     
         19 . An antibody which specifically binds a protein of  claim 1 .  
     
     
         20 . A method for using an antibody to detect protein expression in a sample, the method comprising: 
 a) combining the antibody of  claim 19  with a sample under conditions to form antibody:protein complexes, and    b) detecting complex formation with standards, wherein detection indicates expression of the protein in the sample.