Analysis of gene family expression

Abstract

The present invention relates to methods and compositions for characterizing the expression patterns of genes and gene families. Specifically, the present invention provides means to generate and monitor gene expression profiles resulting from cellular and physiological changes such that the expression patterns of individual genes or groups of genes can be readily identified and characterized.

We claim:  
     
         1 . A method, comprising: 
 a) providing: i) a sample containing nucleic acid, ii) a first primer having a sequence of which at least a portion is at least partially complementary to a first conserved region of a gene from a multigene family, iii) a second primer having a sequence of which at least a portion is at least partially complementary to a second conserved region from said gene of said multigene family, said first and second conserved regions separated in each gene by a distance, said distance varying between a minimum distance and a maximum distance, said maximum distance differing by said minimum distance by less than 500 bases and iv) a polymerase and PCR reagents;    b) preparing said nucleic acid from said sample under conditions so as to produce amplifiable nucleic acid;    c) amplifying said nucleic acid with said first and second primers, said polymerase and said PCR reagents under conditions such that amplified product is generated; and    d) isolating amplified product in a size range, said range defined by a lower end and a higher end, said lower end defined by approximately said minimum distance and said higher end defined by approximately said maximum distance.    
     
     
         2 . The method of  claim 1 , wherein said maximum distance is less than 2000 bases.  
     
     
         3 . The method of  claim 1 , wherein said maximum distance differs from said minimum distance by less than 40% of said maximum distance.  
     
     
         4 . The method of  claim 1 , wherein a portion of said first primer is completely complementary to a first conserved region and a portion of said second primer is completely complementary to a second conserved region.  
     
     
         5 . The method of  claim 1 , wherein, following step (d), said amplified product in said size range is treated with one or more restriction enzymes.  
     
     
         6 . The method of  claim 1 , wherein said preparing of step (b) comprises isolating mRNA.  
     
     
         7 . The method of  claim 6 , wherein said preparing further comprises preparing cDNA from said mRNA.  
     
     
         8 . The method of  claim 1 , wherein said isolating of step (d) comprises electrophoresing said amplified product on a gel.  
     
     
         9 . The method of  claim 8 , wherein said isolating further comprises removing amplified product in said size range from said gel, thereby separating said amplified product in said size range from amplified product not in said size range.  
     
     
         10 . The method of  claim 1 , wherein said gene is from a multigene family selected from the group consisting of protein kinases, phosphatases, ligands, receptors, proteases, cytokines, transmembrane proteins, adapter proteins, G protein-coupled receptors and transcription factors.  
     
     
         11 . A method of analyzing expressed genes in biological samples, comprising: 
 a) providing: i) two samples containing mRNA, ii) a first primer having a sequence of which at least a portion is at least partially complementary to a first conserved region, iii) a second primer having a sequence of which at least a portion is at least partially complementary to a second conserved region, said first and second conserved regions separated in each gene by a distance, said distance varying between a minimum distance and a maximum distance among said genes in said multigene family, said maximum distance differing by said minimum distance by less than 500 bases and, iv) a polymerase and PCR reagents;    b) treating said mRNA of each of said two samples under conditions so as to produce amplifiable DNA from each sample;    c) amplifying said amplifiable DNA from each sample with said first and second primers, said polymerase and said PCR reagents under conditions such that amplified product is generated from each of said two samples;    d) electrophoresing said amplified product from each of said two samples on a gel; and    e) isolating amplified product from each of said two samples in a size range, said range defined by a lower end and a higher end, said lower end defined by approximately said minimum distance and said higher end defined by approximately said maximum distance.    
     
     
         12 . The method of  claim 11 , wherein said maximum distance is less than 2000 bases.  
     
     
         13 . The method of  claim 11 , wherein said maximum distance differs from said minimum distance by less than 40% of said maximum distance.  
     
     
         14 . The method of  claim 11 , wherein a portion of said first primer is completely complementary to a first conserved region and a portion of said second primer is completely complementary to a second conserved region.  
     
     
         15 . The method of  claim 11 , wherein, following step (d), said amplified product in said size range is treated with one or more restriction enzymes.  
     
     
         16 . The method of  claim 11 , wherein said preparing of step (b) comprises isolating mRNA.  
     
     
         17 . The method of  claim 16 , wherein said preparing further comprises preparing cDNA from said mRNA.  
     
     
         18 . The method of  claim 11 , wherein said isolating of step (d) comprises electrophoresing said amplified product on a gel.  
     
     
         19 . The method of  claim 18 , wherein said isolating further comprises removing amplified product in said size range from said gel, thereby separating said amplified product in said size range from amplified product not in said size range.  
     
     
         20 . A kit comprising first and second oligonucleotide primers, said first oligonucleotide primer specific for a first conserved region of a gene of a multigene family and said second oligonucleotide primer specific for a second conserved region of said gene from said multigene family, said first and second conserved regions separated in each gene by a distance.