Method for culturing cell and a culture vessel

Abstract

A method for adhering and proliferating cell, which comprises the steps of inoculating, culturing and then killing fibroblast derived from a mammal, is provided. A culture vessel manufactured according to the steps of the method which can provide improved adhesion to cell and enhanced cell-proliferation is also provided.

What is claimed is:  
     
         1 . A method for adhering and proliferating cell, which comprises the steps of inoculating, culturing and then killing fibroblast derived from a mammal.  
     
     
         2 . The method according to  claim 1 , wherein said killed fibroblast is separated from the culture vessel at least partially.  
     
     
         3 . The method according to  claim 1 , wherein said killed fibroblast is separated from the culture vessel entirely.  
     
     
         4 . The method according to  claim 1 , wherein said fibroblast is killed by at least one treatment selected from the group consisting of freezing, drying and irradiating electromagnetic radiation.  
     
     
         5 . The method according to  claim 4 , wherein electromagnetic radiation is at least one selected from the group consisting of β ray, γ ray, X-ray, electron beam and UV ray.  
     
     
         6 . The method according to  claim 1 , wherein said fibroblast is killed by repeating one treatment selected from the group consisting of freezing, drying and irradiating electromagnetic radiation.  
     
     
         7 . The method according to  claim 6 , wherein electromagnetic radiation is at least one selected from the group consisting of β ray, γ ray, electron beam, UV ray and X-ray.  
     
     
         8 . The method according to  claim 1 , wherein said fibroblast is killed by a combination of at least two treatments selected from the group consisting of freezing, drying and irradiating electromagnetic radiation.  
     
     
         9 . The method according to  claim 8 , wherein electromagnetic radiation is at least one selected from the group consisting of β ray, γ ray, electron beam, UV ray and X-ray.  
     
     
         10 . The method according to  claim 1 , wherein said fibroblast is 3T3 mouse lung fibroblast.  
     
     
         11 . The method according to  claim 1 , wherein said cell is epithelial cell.  
     
     
         12 . The method according to  claim 11 , in which said epithelial cell is epidermal cell.  
     
     
         13 . The method according to  claim 1 , wherein said cell is hepatic cell.  
     
     
         14 . An epidermal cell sheet prepared from the epidermal cell which is cultured using the method according to  claim 1 .  
     
     
         15 . An epidermal cell suspension prepared from the epidermal cell which is cultured using the method according to  claim 1 .  
     
     
         16 . A culture vessel manufactured according to the steps of  claim 1 , which can provide improved adhesion to at least one selected from the group consisting of epithelial and hepatic cells and enhanced cell-proliferation of at least one selected from the group consisting of epithelial and hepatic cells.  
     
     
         17 . The culture vessel according to  claim 16 , wherein said killed fibroblasts are separated from the vessel at least partially.  
     
     
         18 . The culture vessel according to  claim 16 , wherein said killed fibroblasts are separated from the vessel entirely.  
     
     
         19 . The culture vessel according to  claim 16 , which can be preserved by a treatment selected from the group consisting of freezing and drying.  
     
     
         20 . The culture vessel according to  claim 16 , wherein said fibroblast derived from a mammal is 3T3 mouse lung fibroblast.  
     
     
         21 . The culture vessel according to  claim 16 , wherein said epithelial cell is epidermal cell.  
     
     
         22 . The culture vessel according to  claim 16 , which is made of a material selected from the group consisting of glass, synthetic polymer and biopolymer.  
     
     
         23 . The culture vessel according to  claim 16 , which has a shape selected from the group consisting of flask, petri dish, roller bottle, tray, well plate, beads, film, sheet and sponge.  
     
     
         24 . The culture vessel according to  claim 22 , which is made of glass and has a shape selected from the group consisting of flask, petri dish, roller bottle, tray, well plate, beads, film, sheet and sponge.  
     
     
         25 . The culture vessel according to  claim 22 , which is made of synthetic polymer and has a shape selected from the group consisting of flask, petri dish, roller bottle, tray, well plate, beads, film, sheet and sponge.  
     
     
         26 . The culture vessel according to  claim 22 , which is made of biopolymer and has a shape selected from the group consisting of sheet, film, sponge and beads.  
     
     
         27 . The culture vessel according to  claim 16 , whereby an epidermal cell sheet can be prepared.  
     
     
         28 . The culture vessel according to  claim 16 , whereby an epidermal cell suspension can be prepared.