Method for inducing an anti-tumor and anti-cachexia immune response in mammals

Abstract

The invention relates to inducing an immune response toward tumor associated antigens and in particular to the administration of high molecular weight isolates of autologous urine either alone, with adjuvants, or with antigen presenting cells. The antigen presenting cells have been cocultured with isolates of autologous urine. The invention can also be used to treat cachexia in cancer or AIDS patients.

What is claimed is:  
     
         1 . A method for treating cancer in a mammal, comprising: 
 isolating an urine isolate comprising molecules larger than about 1000 daltons from a mammal with cancer; and    administering an effective amount of the urine isolate to said mammal with cancer.    
     
     
         2 . The method of  claim 1  wherein said cancer is a solid tumor.  
     
     
         3 . The method of  claim 1  wherein said administration is by injection.  
     
     
         4 . The method of  claim 3  wherein said injection is selected from the group consisting of intramuscularly, intravenously, intradermally, subcutaneously, and intralymphatically.  
     
     
         5 . The method of  claim 3  wherein said injection is into the tumor itself.  
     
     
         6 . The method of  claim 1  wherein said mammal is a human.  
     
     
         7 . The method of  claim 1  wherein said isolation of said urine isolate comprises: collecting about 1000 to about 10,000 mls of urine from said mammal with cancer, filtering the urine to remove particulate matter, and concentrating the proteins larger than about 1000 daltons.  
     
     
         8 . The method of  claim 6 , wherein said concentrating comprises placing the filtered urine in a concentrator equipped with a 1000 dalton filter cartridge.  
     
     
         9 . The method of  claim 1 , wherein said urine isolate comprises molecules larger than about 5,000 daltons.  
     
     
         10 . The method of  claim 1 , wherein said urine isolate comprises molecules larger than about 10,000 daltons.  
     
     
         11 . The method of  claim 10 , wherein said urine isolate comprises molecules smaller than about 60,000 daltons.  
     
     
         12 . The method of  claim 1 , wherein said urine isolate comprises molecules larger than about 100,000 daltons.  
     
     
         13 . The method of  claim 1 , wherein said urine isolate comprises molecules larger than about 1000 and smaller than about 1,000,000 daltons.  
     
     
         14 . The method of  claim 1 , wherein said urine isolate comprises molecules larger than about 3000 and smaller than about 100,000 daltons.  
     
     
         15 . The method of  claim 1 , wherein said urine isolate comprises molecules larger than about 10,000 and smaller than about 50,000 daltons.  
     
     
         16 . The method of  claim 1 , further comprising administering an effective amount of an immune-stimulating compound.  
     
     
         17 . The method of  claim 16  wherein said immune-stimulating compound is an adjuvant.  
     
     
         18 . The method of  claim 16  wherein said immune-stimulating compound is a heat shock protein.  
     
     
         19 . The method of  claim 16  wherein said immune-stimulating compound is a bacterial cell wall extract.  
     
     
         20 . A method for treating cachexia in a mammal, comprising: 
 isolating a urine isolate comprising molecules larger than about 1000 daltons from a mammal with cancer; and    administering an effective amount of the sterile urine isolate to a mammal with cachexia.    
     
     
         21 . The method of  claim 20  wherein said cancer is a solid tumor.  
     
     
         22 . The method of  claim 20  wherein said administration is by injection.  
     
     
         23 . The method of  claim 22  wherein said injection is selected from the group consisting of intramuscularly, intravenously, intradermally, subcutaneously, and intralymphatically.  
     
     
         24 . The method of  claim 22  wherein said injection is into the tumor itself.  
     
     
         25 . The method of  claim 20  wherein said mammal is a human.  
     
     
         26 . The method of  claim 20  wherein said isolation of said sterile urine isolate comprises: collecting about 1000 to about 10,000 mls of urine from said mammal with cancer, filtering the urine to remove particulate matter, and concentrating the proteins larger than about 1000 daltons.  
     
     
         27 . The method of  claim 26 , wherein said concentrating comprises placing the filtered urine in a concentrator equipped with a 1000 dalton filter cartridge.  
     
     
         28 . The method of  claim 20  wherein said cachexia is due to cancer or AIDS.  
     
     
         29 . The method of  claim 20 , wherein said urine isolate comprises molecules larger than about 10,000 daltons and smaller than about 100,000 daltons.  
     
     
         30 . The method of  claim 20 , wherein said urine isolate comprises molecules larger than about 10,000 daltons and smaller than about 40,000 daltons.  
     
     
         31 . The method of  claim 20 , further comprising administering an effective amount of an immune-stimulating compound.  
     
     
         32 . The method of  claim 31  wherein said immune-stimulating compound is an adjuvant.  
     
     
         33 . The method of  claim 31  wherein said immune-stimulating compound is a heat shock protein.  
     
     
         34 . The method of  claim 31  wherein said immune-stimulating compound is a bacterial cell wall extract.  
     
     
         35 . A method for the treatment of cancer in a mammal, comprising: isolating a urine isolate comprising molecules larger than about 1000 daltons from a mammal with cancer; and co-culturing antigen-presenting cells (APCs) with the urine isolate; and reinfusing an effective amount of the APCs or exosomes from the APCs into the mammal with cancer.  
     
     
         36 . The method of  claim 35  wherein said APCs are isolated from the mammal with cancer.  
     
     
         37 . The method of  claim 35  wherein said APCs are dendritic cells.  
     
     
         38 . The method of  claim 35  wherein said cancer is a solid tumor.  
     
     
         39 . The method of  claim 35  wherein said reinfusion is by injection.  
     
     
         40 . The method of  claim 39  wherein said injection is selected from the group consisting of intramuscularly, intravenously, intradermally, subcutaneously, and intralymphatically.  
     
     
         41 . The method of  claim 39  wherein said injection is into the tumor itself.  
     
     
         42 . The method of  claim 35  wherein said mammal is a human.  
     
     
         43 . The method of  claim 35  wherein said isolation of said sterile urine isolate comprises: collecting about 1000 to about 10,000 mls of urine from said mammal with cancer, filtering the urine to remove particulate matter, and concentrating the proteins larger than about 1000 daltons.  
     
     
         44 . The method of  claim 35 , wherein said concentrating comprises placing the filtered urine in a concentrator equipped with a 1000 dalton filter cartridge.  
     
     
         45 . The method of  claim 35 , wherein said urine isolate comprises molecules larger than about 10,000 daltons and smaller than about 100,000 daltons.  
     
     
         46 . The method of  claim 45 , wherein said urine isolate comprises molecules larger than about 40,000 daltons and smaller than about 100,000 daltons.  
     
     
         47 . The method of  claim 46 , wherein said urine isolate comprises molecules smaller than about 60,000 daltons and smaller than about 100,000 daltons.  
     
     
         48 . The method of  claim 45 , wherein said urine isolate comprises molecules larger than about 10,000 daltons and smaller than about 40,000 daltons.  
     
     
         49 . The method of  claim 35 , further comprising administering an effective amount of an immune-stimulating compound.  
     
     
         50 . The method of  claim 49  wherein said immune-stimulating compound is an adjuvant.  
     
     
         51 . The method of  claim 48  wherein said immune-stimulating compound is a heat shock protein.  
     
     
         52 . The method of  claim 48  wherein said immune-stimulating compound is a bacterial cell wall extract.  
     
     
         53 . A method for treating cachexia in mammals which comprises: 
 isolating molecules larger than about 1000 daltons from the urine of a mammal with cancer;    co-culturing APCs with the urine isolate; and    reinfusing an effective amount of the APCs into the mammal with cachexia.    
     
     
         54 . The method of  claim 53  wherein said APCs are isolated from the mammal with cancer.  
     
     
         55 . The method of  claim 53  wherein said APCs are dendritic cells.  
     
     
         56 . The method of  claim 53  wherein said cancer is a solid tumor.  
     
     
         57 . The method of  claim 53  wherein said reinfusion is by injection.  
     
     
         58 . The method of  claim 57  wherein said injection is selected from the group consisting of intramuscularly, intravenously, intradermally, subcutaneously, and intralymphatically.  
     
     
         59 . The method of  claim 53  wherein said injection is into the tumor itself.  
     
     
         60 . The method of  claim 53  wherein said mammal is a human.  
     
     
         61 . The method of  claim 53  wherein said isolation of said sterile urine isolate comprises: collecting about 1000 to about 10,000 mls of urine from said mammal with cancer, filtering the urine to remove particulate matter, and concentrating the proteins larger than about 1000 daltons.  
     
     
         62 . The method of  claim 61 , wherein said concentrating comprises placing the filtered urine in a concentrator equipped with a 1000 dalton filter cartridge.  
     
     
         63 . The method of  claim 53  wherein said cachexia is due to cancer or AIDS.  
     
     
         64 . The method of  claim 53 , wherein said urine isolate comprises molecules larger than about 10,000 daltons and smaller than about 100,000 daltons.  
     
     
         65 . The method of  claim 53 , wherein said urine isolate comprises molecules larger than about 10,000 daltons and smaller than about 40,000 daltons.  
     
     
         66 . The method of  claim 53 , further comprising administering an effective amount of an immune-stimulating compound.  
     
     
         67 . The method of  claim 66  wherein said immune-stimulating compound is an adjuvant.  
     
     
         68 . The method of  claim 66  wherein said immune-stimulating compound is a heat shock protein.  
     
     
         69 . The method of  claim 66  wherein said immune-stimulating compound is a bacterial cell wall extract.