Expression and secretion of heterologous polypeptides from caulobacter

Abstract

DNA constructs are provided which code for at least the extreme C-terminal amino acids of the rsa A protein of Caulobacter crescentus fused with heterologous polypeptides. Baterial cells containing, or which express the DNA constructs and secrete the resulting protein are also provided. Chimeric proteins including the C-terminal amino acids of the rsa A protein are provided, including chimeric proteins comprising antigenic epitopes of the Infectious Hematopoietic Necrosis Virus.

We claim:  
     
         1 . A DNA construct comprising in sequence, one or more restriction sites for facilitating insertion of DNA to the construct and, DNA encoding at least the C-terminal region of the  rsa A protein of  C. crescentus,  wherein the C-terminal region comprises at least amino acids 944-1026 of the  rsa A protein.