Stabilized aqueous nucleoside triphosphate solution

Abstract

The invention concerns stable aqueous solutions containing one or several nucleoside triphosphates wherein the respective solution has a pH value of more than 7.5 and contains no additional substances with a stabilizing effect. The nucleoside triphosphate solutions are used in particular for DNA synthesizing reactions such as e.g. RT-PCR, cycle sequencing, random priming and nick translation. One of the most important applications of such solutions containing deoxynucleoside triphosphates (d-NTP) is their use in the polymerase chain reaction (PCR).

1 . Stable aqueous solution containing nucleoside triphosphates, wherein the pH value of the solution is above ca. 7.5.  
     
     
         2 . Stable aqueous solution as claimed in  claim 1 , wherein the nucleoside triphosphates are modified nucleoside triphosphates.  
     
     
         3 . Stable aqueous solution as claimed in  claim 1  or  2 , wherein the pH value is in a range between 7.5 and 11.  
     
     
         4 . Stable aqueous solution as claimed in  claim 1 ,  2  or  3 , wherein the concentration of the nucleoside triphosphates is ca. 2 to 200 mmol/l.  
     
     
         5 . Stable aqueous solution as claimed in one of the  claims 1  to  4 , wherein the solution contains deoxynucleoside triphosphates.  
     
     
         6 . Stable aqueous solution as claimed in  claims 1  to  5  containing a substance which buffers at or above pH 7.5.  
     
     
         7 . Stable aqueous solution as claimed in  claims 1  to  6  which are free of further stabilizing agents.  
     
     
         8 . Use of a stable aqueous solution as claimed in  claims 1  to  7  for a DNA and/or RNA synthesizing reaction.  
     
     
         9 . Use of a stable aqueous solution as claimed in  claims 1  to  7  to replicate DNA and/or RNA sequences or fragments.  
     
     
         10 . Use of a stable aqueous solution as claimed in  claims 1  to  7  to specifically replicate nucleic acid fragments in the presence of an enzyme with reverse transcriptase activity.  
     
     
         11 . Use of a stable aqueous solution as claimed in  claims 1  to  7  for the cycle sequencing of nucleic acids.  
     
     
         12 . Use of a stable aqueous solution as claimed in  claims 1  to  7  for the specific replication of deoxynucleic acid sequences or fragments.  
     
     
         13 . Use of a stable aqueous solution as claimed in  claims 1  to  7  for random priming.  
     
     
         14 . Use of a stable aqueous solution as claimed in  claims 1  to  7  for nick translation.