Method of separating different chemicals in a sample

Abstract

Disclosed is a method for separating a plurality of different chemicals in a sample. The method uses a hexyl-bonded stationary phase composition in reversed-phase liquid chromatography. The composition includes a polymer-based chromatographic support composed of chemically stable and porous particles having a surface. The surface of said particles having hexyl-phenyl bonded thereto. A chromatography column is packed with this composition and a sample including a plurality of different chemicals is injected. Liquid is applied to force the sample through the column, whereby said different chemicals come out of the column at different times.

What is claimed is:  
     
         1 . A method for separating a plurality of different chemicals in a sample comprising said different chemicals, said method comprising the steps of: 
 preparing a stationary phase composition comprising a polymer-based chromatographic support which comprises chemically stable and porous particles having a surface, the surface of said particles having hexyl-phenyl bonded thereto;    packing said polymer-based chromatographic support inside a column;    injecting said sample comprising a plurality of different chemicals into a first end of the column; and    applying liquid flow into said first end of said column, thereby forcing said sample through said column and out of a second end of said column, whereby said different chemicals come out of said second end of said column at different times.    
     
     
         2 . The method as defined in    claim 1   , wherein said polymer-based chromatographic support is one selected from the group consisting of silica gel, polystyrene-divinylbenzene copolymers, polyhydroxymethacrylate, and cellulose.  
     
     
         3 . The composition of    claim 2   , wherein said polymer-based chromatographic support is silica gel.  
     
     
         4 . The method as defined in    claim 3   , wherein the preparation of the polymer-based chromatographic support comprises reacting silica gel with a reactive hexyl-phenylsilane.  
     
     
         5 . The method as defined in    claim 1   , wherein the surface of the particles has at least one additional alkyl-phenyl bonded thereto, wherein said alkyl-phenyl has from five to ten methylene groups in the alkyl group.  
     
     
         6 . The method as defined in    claim 5   , wherein the alkyl-phenyl comprises octyl-phenyl.  
     
     
         7 . A method of using a composition in reversed-phase liquid chromatography as stationary phase to separate a plurality of different chemicals in a sample comprising said different chemicals, the composition comprising a polymer-based chromatographic support which comprises chemically stable and porous particles having a surface, the surface of said particles having hexyl-phenyl bonded thereto.  
     
     
         8 . The method as defined in    claim 7   , wherein said polymer-based chromatographic support is one selected from the group consisting of silica gel, polystyrene-divinylbenzene copolymers, polyhydroxymethacrylate, and cellulose.  
     
     
         9 . The composition of    claim 8   , wherein said polymer-based chromatographic support is silica gel.  
     
     
         10 . The method as defined in    claim 9   , wherein the preparation of the polymer-based chromatographic support comprises reacting silica gel with a reactive hexyl-phenylsilane.  
     
     
         11 . The method as defined in    claim 7   , wherein the surface of the particles has at least one additional alkyl-phenyl bonded thereto, wherein said alkyl-phenyl has from five to ten methylene groups in the alkyl group.  
     
     
         12 . The method as defined in    claim 11   , wherein the alkyl-phenyl comprises octyl-phenyl.