Laboratory in a disk

Abstract

An apparatus is described that includes an optical disk, adapted to be read by an optical reader, comprising a first sector having substantially self-contained assay means for localizing an analyte suspected of being in a sample to at least one, predetermined location in the first sector and a second sector containing control means for conducting the assay and analyte location information, with respect to one or more analytes suspected of being in a sample, accessible to the reader, wherein the presence or absence of the analyte at said location is determinable by the reader using the control means and the location information. Depending on the nature of the assay, the disk will include fluid storage means, fluid transfer means, such as one or more capillary ducts, valves, batteries, dialyzers, columns, filters, sources of electric fields, wires or other electrical conductive means such as metallic surface deposits and the like.

What is claimed is:  
     
         1 . An optical disk, adapted to be read by an optical reader, comprising a first sector having a substantially self-contained assay means for binding or reacting an analyte suspected of being in a sample to at least one, predetermined location in the first sector and optionally a second sector containing a control means for conducting the assay and analyte location information with respect to one or more analytes suspected of being in a sample, accessible to a reader, and wherein the presence or absence of the analyte at said location is determinable by the reader using the control means and the location information.  
     
     
         2 . The optical disk of    claim 1    which includes a sealable sample entry port in fluid communication with the assay means.  
     
     
         3 . An apparatus for conducting an assay comprising an optical disk, a disk reader and an information processor, wherein the disk comprises a first sector having a substantially self-contained assay means for binding an analyte suspected of being in a sample to at least one, predetermined location in the first sector and optionally a second sector containing control information for conducting the assay and analyte location information with respect to one or more analytes suspected of being in the sample, accessible to the reader and processable by the information processor, wherein the disk is adapted to be read by the reader and the information processor is adapted to determine the presence or absence of the analyte at said location using the control information and the location information.  
     
     
         4 . The apparatus of    claim 3    wherein the reader is a CD-ROM or a DVD reader and wherein the reader is adapted to be coupled to an information processor.  
     
     
         5 . The apparatus of    claim 4    wherein the information processor is a personal computer.  
     
     
         6 . The disk of    claim 1    wherein the assay means comprises a fluid storage means and a fluid transfer means formed in a disk surface.  
     
     
         7 . The disk of    claim 6    wherein the fluid transfer means comprises a capillary duct.  
     
     
         8 . The disk of    claim 7    wherein the fluid transfer means comprises a valve.  
     
     
         9 . The disk of    claim 6    wherein the disk comprises an electrochemical energy means.  
     
     
         10 . The disk of    claim 1    wherein the assay means comprises a sample port, a sample preparation sector, an analyte separation sector and an assay sector wherein the analyte is localized.  
     
     
         11 . The disk of    claim 6    wherein the fluid transfer means is responsive to centrifugal force or an electric field.  
     
     
         12 . The disk of    claim 1    wherein the disk comprises a multiplicity of first sectors adapted to analyze for a multiplicity of analytes.  
     
     
         13 . The disk of    claim 1    further comprising a multiplicity of first sectors adapted to analyze for the same analyte or different analytes wherein each of said multiplicity of sectors is adapted for fluid communication to a sample port.  
     
     
         14 . An assay element comprising a substrate, a first oligonucleotide bound to the substrate, a spacer molecule bound at a first end to the first oligonucleotide by a second oligonucleotide that is complementary to the first oligonucleotide, wherein the spacer molecule further comprises a means for binding to an analyte in a sample and having a second end that is detectable by a detection means, the spacer molecule further comprising a site intermediate the first and second ends that is cleavable, the binding means having a first moiety between the first end of the spacer molecule and the cleavage site for binding to a first part of the analyte and a second moiety between the second end of the spacer molecule and the cleavage site for binding to a second part of the analyte.  
     
     
         15 . An assay component, adapted to be read by a CD-ROM or a DVD reader comprising an optical disk and a substantially self-contained assay means in the disk for binding an analyte suspected of being in a sample to at least one, predetermined location on the disk and means at said location for enabling detection of the absence or presence of the analyte by the CD-ROM or the DVD reader.  
     
     
         16 . An optical disk, adapted to be read by a CD-ROM or a DVD reader comprising a substantially self-contained assay means for localizing an analyte suspected of being in a sample to at least one, predetermined location on the disk and a means at said location for detecting the absence or presence of the analyte by the CD-ROM or the DVD reader.