Chromatin associated proteins

Abstract

This invention relates to an isolated nucleic acid fragment encoding a chromatin associated protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the chromatin associated protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the chromatin associated protein in a transformed host cell.

What is claimed is:  
     
         1 . An isolated nucleic acid fragment encoding a histone deacetylase 3 protein comprising a member selected from the group consisting of: 
 (a) an isolated nucleic acid fragment encoding the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO: 2, 4, 6 and 8;    (b) an isolated nucleic acid fragment that is complementary to (a).    
     
     
         2 . The isolated nucleic acid fragment of    claim 1    wherein nucleic acid fragment is a functional RNA.  
     
     
         3 . The isolated nucleic acid fragment of    claim 1    wherein the nucleotide sequence of the fragment comprises the sequence set forth in a member selected from the group consisting of SEQ ID NO: 1, 3, 5 and 7.  
     
     
         4 . A chimeric gene comprising the nucleic acid fragment of    claim 1    operably linked to suitable regulatory sequences.  
     
     
         5 . A transformed host cell comprising the chimeric gene of    claim 4   .  
     
     
         6 . A histone deacetylase 3 polypeptide comprising the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO: 2, 4, 6 and 8.  
     
     
         7 . A method of altering the level of expression of a chromatin associated protein in a host cell comprising: 
 (a) transforming a host cell with the chimeric gene comprising a nucleic acid fragment that encodes an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO: 2, 4, 6 and 8; and    (b) growing the transformed host cell produced in step (a) under conditions that are suitable for expression of the chimeric gene    wherein expression of the chimeric gene results in production of altered levels of a chromatin associated protein in the transformed host cell.    
     
     
         8 . A method of obtaining a nucleic acid fragment encoding all or a substantial portion of the amino acid sequence encoding a chromatin associated protein comprising: 
 (a) probing a cDNA or genomic library with the nucleic acid fragment of    claim 1   ;    (b) identifying a DNA clone that hybridizes with the nucleic acid fragment of    claim 1   ;    (c) isolating the DNA clone identified in step (b); and    (d) sequencing the cDNA or genomic fragment that comprises the clone isolated in step (c)    wherein the sequenced nucleic acid fragment encodes all or a substantial portion of the amino acid sequence encoding a chromatin associated protein.    
     
     
         9 . A method of obtaining a nucleic acid fragment encoding a substantial portion of an amino acid sequence encoding a chromatin associated protein comprising: 
 (a) synthesizing an oligonucleotide primer corresponding to a portion of the sequence set forth in any of SEQ ID NOs: 1, 3, 5 and 7; and    (b) amplifying a cDNA insert present in a cloning vector using the oligonucleotide primer of step (a) and a primer representing sequences of the cloning vector    wherein the amplified nucleic acid fragment encodes a substantial portion of an amino acid sequence encoding a chromatin associated protein.    
     
     
         10 . The product of the method of    claim 8   .  
     
     
         11 . The product of the method of    claim 9   .