US2001007752A1PendingUtilityA1

Agouti-related transcript antibodies and methods of use

Assignee: AMGEN INCPriority: May 10, 1996Filed: Jan 5, 2001Published: Jul 12, 2001
Est. expiryMay 10, 2016(expired)· nominal 20-yr term from priority
Inventors:Kevin Stark
A61K 38/00A61P 43/00C07K 14/47
54
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Claims

Abstract

Disclosed is a novel gene termed ART which is expressed primarily in selected regions of the brain, as well as adrenal and lung tissues. Polypeptides encoded by ART are also disclosed, as are methods for preparing ART DNA and amino acid sequences.

Claims

exact text as granted — not AI-modified
We claim:  
     
         1 . A nucleic acid molecule encoding a polypeptide selected from the group consisting of: 
 (a) the nucleic acid molecule of SEQ ID NO:4;    (b) the nucleic acid molecule of SEQ ID NO:5;    (c) the nucleic acid molecule of SEQ ID NO:6;    (d) the nucleic acid molecule of SEQ ID NO:9    (e) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:8;    (f) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:10;    (g) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:11;    (h) a nucleic acid molecule that encodes a polypeptide that is at least 70 percent identical to the polypeptides of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:11; and    (i) a nucleic acid molecule that is the complement of any of (a)-(h) above.    
     
     
         2 . The nucleic acid molecule that is SEQ ID NO:4.  
     
     
         3 . The nucleic acid molecule that is SEQ ID NO:5.  
     
     
         4 . The nucleic acid molecule of SEQ ID NO:6.  
     
     
         5 . The nucleic acid molecule of SEQ ID NO:9.  
     
     
         6 . A nucleic acid molecule encoding the polypeptide of SEQ ID NO:7.  
     
     
         7 . A nucleic acid molecule encoding the polypeptide of SEQ ID NO:8.  
     
     
         8 . A nucleic acid molecule encoding the polypeptide of SEQ ID NO:10.  
     
     
         9 . A nucleic acid molecule encoding the polypeptide of SEQ ID NO:11.  
     
     
         10 . A vector comprising the nucleic acid molecule of    claim 1   .  
     
     
         11 . A vector comprising the nucleic acid molecule of    claim 2   .  
     
     
         12 . A vector comprising the nucleic acid molecule of    claim 3   .  
     
     
         13 . A vector comprising the nucleic acid molecule of    claim 4   .  
     
     
         14 . A vector comprising the nucleic acid molecule of    claim 5   .  
     
     
         15 . A vector comprising the nucleic acid molecule of    claim 6   .  
     
     
         16 . A vector comprising the nucleic acid molecule of    claim 9   .  
     
     
         17 . A host cell comprising the vector of    claim 10   .  
     
     
         18 . A host cell comprising the vector of    claim 11   .  
     
     
         19 . A host cell comprising the vector of    claim 12   .  
     
     
         20 . A host cell comprising the vector of    claim 13   .  
     
     
         21 . A host cell comprising the vector of    claim 14   .  
     
     
         22 . A host cell comprising the vector of    claim 15   .  
     
     
         23 . A host cell comprising the vector of    claim 16   .  
     
     
         24 . A process for producing an ART polypeptide comprising the steps of: 
 (a) expressing a polypeptide encoded by the nucleic acid of    claim 1    in a suitable host; and    (b) isolating the polypeptide.    
     
     
         25 . The process of    claim 19    wherein the polypeptide is selected from the group consisting of: SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:11.  
     
     
         26 . An ART polypeptide selected from the group consisting of: 
 (a) the polypeptide of SEQ ID NO:7;    (b) the polypeptide of SEQ ID NO:8;    (c) the polypeptide of SEQ ID NO:10;    (d) the polypeptide of SEQ ID NO:11; and    (e) a polypeptide that is 70 percent homologous with the polypeptide of (a) or (b).    
     
     
         27 . An ART polypeptide that is the polypeptide of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:11, or a biologically active fragment thereof.  
     
     
         28 . An ART polypeptide that is at least 70 percent identical to the ART polypeptide of    claim 27   .  
     
     
         29 . The ART polypeptide of    claim 27    that does not possess an amino terminal methionine.  
     
     
         30 . The ART polypeptide of    claim 27    that possesses an amino terminal methionine.  
     
     
         31 . A method of increasing food uptake in a mammal comprising administering the polypeptide of    claim 27    to the mammal.

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