Promoter

Abstract

The present invention provides a DNA having promoter activity of Tec tyrosine kinase and a vector having incorporated within it the promoter to thereby enable a high level expression of an exogenous gene in hematopoietic stem cells and hepatic cells.

1 . An isolated DNA having promoter activity and comprising a part of the nucleotide sequence of SEQ ID No: 9, wherein the part has a length of at least about 50 bp and wherein the promoter activity is associated with the part.  
     
     
         2 . The isolated DNA of    claim 1   , wherein the part has a length of at least about 100 bp.  
     
     
         3 . The isolated DNA of    claim 1   , wherein the part has a length of at least about 150 bp.  
     
     
         4 . The isolated DNA of    claim 1   , wherein the part has a length of at least about 200 bp.  
     
     
         5 . The isolated DNA of    claim 1   , wherein the part has a length of at least about 300 bp.  
     
     
         6 . The isolated DNA of    claim 1   , wherein the part has a length of at least about 400 bp.  
     
     
         7 . The isolated DNA of    claim 1   , wherein the part has a length of at least about 500 bp.  
     
     
         8 . An isolated DNA having promoter activity consisting essentially of SEQ ID No: 9 wherein the promoter activity is associated with SEQ ID No: 9.  
     
     
         9 . An expression vector comprising the isolated DNA of    claim 1   .  
     
     
         10 . A cell comprising the expression vector of    claim 9   .