Multicomponent clostridial vaccines using saponin adjuvants

Abstract

Novel multicomponent clostridial vaccine formulations using readily dispersible, non-depot adjuvants are disclosed. The vaccines can be administered to cattle intramuscularly or subcutaneously, without the severe, persistent local reactions, such as granulomas, abscesses and scarring, normally seen with other multicomponent clostridial vaccines.

1 . A multicomponent clostridial vaccine composition comprising two or more clostidial immunogens and a rapidly dispersed adjuvant.  
     
     
         2 . The vaccine composition of    claim 1   , wherein said adjuvant is saponin.  
     
     
         3 . The vaccine composition of    claim 1    wherein said clostridial immunogens comprise two or more clostridial bacterins or toxoids.  
     
     
         4 . The vaccine composition of    claim 3    wherein said two or more clostridial bacterins or toxoids are derived from the group of Clostridia consisting of  Clostridium perfringens, Clostridium septicum, Clostridium tetani, Clostridium chauvoei, Clostridium novyi, Clostridium sordellii, Clostridium haemolyticum, Clostridium botulinum,  and serotypes thereof.  
     
     
         5 . The vaccine composition of    claim 3    wherein said composition comprises clostridial bacterins or toxoids derived from each of  Clostridium chauvoei, Clostridium septicum, Clostridium novyi, Clostridium sordellii, Clostridium perfringens,  Type C and  Clostridium perfringens,  Type D.  
     
     
         6 . The vaccine composition of    claim 3    wherein said composition comprises clostridial bacterins or toxoids derived from each of  Clostridiun haemolyticum, Clostridium chauvoei, Clostridiun-septicum, Clostrtdium novyi, Clostridiwn sordellii, Clostridium perfringens,  Type C and  Clostridium perfringens,  Type D.  
     
     
         7 . A multicomponent clostridial vaccine composition comprising: 
 (a) clostridial bacterins or toxoids derived from each of  Clostridiun chauvoei, Clostridium septicum, Clostridium novyi, Clostridium sordellii, Clostridiun perfringens,  Type C and  Clostridium perfringens,  Type D; and    (b) a saponin adjuvant.    
     
     
         8 . A multicomponent clostridial vaccine composition comprising: 
 (a) clostridial bacterins or toxoids derived from each of  Clostridiwn haemolyticum, Clostridium chauvoei, Clostridium septicun, Clostridium novyi, Clostridiun sordellii, Clostridiun perfringens,  Type C and  Clostridiwn perfringens,  Type D; and    (b) a saponin adjuvant.    
     
     
         9 . A method of preventing or treating clostridial infection in a bovine animal, said method comprising administering an effective amount of the vaccine composition of    claim 1    to said bovine animal.  
     
     
         10 . A method of preventing or treating clostridial infection in a bovine animal, said method comprising administering an effective amount of the vaccine composition of    claim 7    to said bovine animal.  
     
     
         11 . A method of preventing or treating clostridial infection in a bovine animal, said method comprising administering an effective amount of the vaccine composition of    claim 8    to said bovine animal.  
     
     
         12 . The method of    claim 9    wherein said administering is done via an intramuscular injection.  
     
     
         13 . The method of    claim 10    wherein said administering is done via an intramuscular injection.  
     
     
         14 . The method of    claim 10    wherein said administering is done via an intramuscular injection.  
     
     
         15 . The method of    claim 9    wherein said administering is done via a subcutaneous injection.  
     
     
         16 . The method of    claim 10    wherein said administering is done via a subcutaneous injection.  
     
     
         17 . The method of    claim 11    wherein said administering is done via a subcutaneous injection.