Method of isolating myogenic progenitors using CD54

Abstract

Provided herein are isolated myogenic progenitor cells, methods to isolate myogenic progenitor cells and method of their use.

What is claimed is: 
     
         1 . A method for isolating myogenic progenitor cells, wherein said method comprises:
 (a) contacting isolated mammalian pluripotent stem cells comprising an exogenous nucleic acid sequence encoding PAX7 operably linked to an inducible promoter with myogenic differentiation medium and a compound that induces PAX7 expression such that myogenic progenitor cells that express CD54 are obtained;   (b) separating the myogenic progenitor cells that express CD54 obtained in step (a); and   (c) culturing myogenic progenitor cells that express CD54 separated in step (b).   
     
     
         2 . The method of  claim 1 , wherein the pluripotent stem cells are induced pluripotent stem (iPS) cells. 
     
     
         3 . The method of  claim 1 , wherein the separating contacting is performed using Fluorescent Activated Cell Sorting (FACS). 
     
     
         4 . A method of treating injured or diseased muscle tissue in a mammal, the method comprising: administering the myogenic progenitor cells that express CD54 cultured in step (c) of  claim 1  into injured or diseased muscle tissue of a mammal such that the muscle tissue is treated. 
     
     
         5 . The method of  claim 4 , wherein the injured or diseased muscle tissue is a result of injury or overuse, a genetic disorder, cancer, inflammation, infection, or medical treatment. 
     
     
         6 . The method of  claim 1 , wherein the pluripotent stem cells are embryonic stem cells. 
     
     
         7 . The method of  claim 1 , wherein the separating is performed using magnetic sorting.