Isolation device and isolation method for isolating target particles from liquid samples

Abstract

An isolation device for isolation of target particles from a plurality of liquid samples includes a plurality of isolation chips and a vacuum system. Each of the plurality of isolation chips includes a sample reservoir, and a first outlet and a second outlet disposed at opposite sides of the sample reservoir. The vacuum system includes a first vacuum pump connected to the first outlet of each of the plurality of isolation chips and a second vacuum pump connected to the second outlet of each of the plurality of isolation chips. The first vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding first outlet. The second vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding second outlet. The target particles are isolated from each of the plurality of liquid samples in a corresponding sample reservoir.

What is claimed is: 
     
       1. An isolation device for isolation of target particles from a plurality of liquid samples, the isolation device comprising:
 a plurality of isolation chips each comprising:
 a sample reservoir configured for receiving one of the plurality of liquid samples; 
 a first outlet and a second outlet disposed at opposite sides of the sample reservoir; 
 
 a vacuum system comprising:
 a first vacuum pump connected to the first outlet of each of the plurality of isolation chips, the first vacuum pump configured to generate a negative pressure in each of the plurality of isolation chips through a corresponding first outlet; and 
 a second vacuum pump connected to the second outlet of each of the plurality of isolation chips, the second vacuum pump configured to generate a negative pressure in each of the plurality of isolation chips through a corresponding second outlet, to isolate the target particles from each of the plurality of liquid samples in a corresponding sample reservoir; 
 
 a first support platform adapted to rotate about a first shaft and configured to support the plurality of isolation chips, the plurality of isolation chips arranged around the first shaft and moved to a first preset position when the first support platform rotates; 
 a second support platform adapted to rotate about a second shaft and configured to support the plurality of liquid samples, the plurality of liquid samples arranged around the second shaft and moved to a second preset position when the second support platform rotates; and 
 a liquid collector comprising at least one sampling member, the at least one sampling member adapted to rotate about a third shaft to form a sampling trajectory, the first preset position and the second preset position disposed on the sampling trajectory, the at least one sampling member configured to collect one of the plurality of liquid samples at the second preset position and add the collected liquid sample to one of the plurality of isolation chips at the first preset position. 
 
     
     
       2. The isolation device of  claim 1 , wherein the at least one sampling member comprises two sampling members, one of the two sampling members is disposed on a side of a line connecting the first support platform and the second support platform, the other one of the two sampling members is disposed on another side of the line connecting the first support platform and the second support platform; each of the two sampling members form the sampling trajectory, respectively;
 two first preset positions and two second preset positions are formed, one of the two first preset positions and a corresponding one of the two second preset positions are disposed on the sampling trajectory of one of the two sampling members, and the other one of the two first preset positions and a corresponding one of the two second preset positions are disposed on the sampling trajectory of the other one of the two sampling members. 
 
     
     
       3. The isolation device of  claim 1 , wherein each of the plurality of isolation chips further comprises:
 a first filtration membrane comprising pores of sizes smaller than sizes of the target particles; 
 a second filtration membrane comprising pores of sizes smaller than the sizes of the target particles; 
 a first chamber connected to the sample reservoir through the first filtration membrane, the first chamber connected to the first outlet, the first chamber communicating with an ambient environment through the first outlet; and 
 a second chamber connected to the sample reservoir through the second filtration membrane, the second chamber connected to the second outlet, the second chamber communicating the ambient environment with the second outlet, the first chamber and the second chamber disposed at the opposite sides of the sample reservoir. 
 
     
     
       4. The isolation device of  claim 1 , further comprising a frequency converting module, wherein the frequency converting module is connected to the first outlet and the second outlet of each of the plurality of isolation chips through the vacuum system. 
     
     
       5. The isolation device of  claim 4 , wherein the frequency converting module comprises a frequency converter and a valve connected to the frequency converter, the valve is alternately switched to connect one of the first vacuum pump and the second vacuum pump, so that the first vacuum pump and the second vacuum pump alternately operate. 
     
     
       6. The isolation device of  claim 3 , wherein the vacuum system is configured to alternately generate the negative pressure in the first chamber and the second chamber of each of the plurality of isolation chips, which is caused by rectangular wave shaped pulse signals, periodic sinusoidal wave shaped pulse signals, or trapezoidal wave shaped pulse signals.