Water-soluble and water-insoluble propolis products with high antioxidant capacity and their production methods

Abstract

Disclosed are propolis products suitable for use in various industries such as food, health and cosmetics where propolis, which promote various beneficial biological activities in addition to having antimicrobial, antifungal, antiviral, anti-inflammatory, anti-cancer and anesthetic affects, is extracted correctly to offer it a form suitable for human consumption and with the highest bioavailability, where it is fast and easy to consume. Also disclosed is a method of production thereof.

The invention claimed is: 
     
       1. A method for producing a propolis product comprising:
 (a) grinding raw propolis into crumbles with a grinder; 
 (b) mixing the crumbles with solvent material, wherein the solvent material is selected from the group consisting of: ethyl alcohol, propylene glycol, glycerol, a mixture of ultra-pure water and mineral water, olive oil, almond oil, linseed oil, fish oil, pomegranate juice, orange juice, beetroot juice, grape juices and mixtures thereof; 
 (c) adding an acid regulator to the mixed crumbles and solvent material to produce an obtained solution, wherein the acid regulator is selected from the group consisting of: lemon juice, apple cider vinegar and mixtures thereof; 
 (d) shaking the obtained solution in a dark environment for at least one week after the acid regulator is added; 
 (e) degassing the shaken obtained solution; 
 (f) centrifuging the degassed solution to produce a supernatant extract of the degassed solution; 
 (g) analyzing phenolic flavonoid matter of the supernatant extract; 
 (h) determining dry matter in the extract; 
 (i) processing the extract to produce the propolis product. 
 
     
     
       2. The method of  claim 1 , wherein the step of mixing comprises:
 mixing 200 to 500 grams of the crumbles with 750 to 1500 grams of the solvent material, the solvent material selected from the group consisting of ethyl alcohol, olive oil, almond oil, linseed oil, fish oil, pomegranate juice, orange juice, beetroot juice, and grape juice. 
 
     
     
       3. The method of  claim 1 , wherein the step of mixing comprises:
 mixing 200 to 500 grams of the crumbles with 800 to 100 grams of the solvent material, the solvent material selected from the group consisting of propylene glycol and glycerol. 
 
     
     
       4. The method of  claim 1 , wherein the step of mixing comprises:
 mixing 200 to 500 grams of the crumbles with 250 to 300 grams of the ultra-pure water and 250 to 1000 grams of mineral water in the weight ratio of 1:1 to 1:3 of the crumbles with the ultra-pure water and the mineral water. 
 
     
     
       5. The method of  claim 1 , wherein the step of mixing comprises:
 mixing 200 to 500 grams of the crumbles with 250 to 300 grams of the ultra-pure water and 250 to 1000 grams of mineral water in the weight ratio of 1:1 to 1:3 of the crumbles with the ultra-pure water and the mineral water. 
 
     
     
       6. The method of  claim 1 , wherein the step of adding the acid regulator comprises:
 mixing 50 to 200 grams of the acid regulator to 200-500 grams of the crumbles. 
 
     
     
       7. The method of  claim 1 , wherein the step of degassing comprises:
 degassing the shaken obtained, solution in an ultrasonic bath for three hours at 25° C. 
 
     
     
       8. The method of  claim 1 , wherein the step of centrifuging comprises:
 centrifuging the degassed solution at 10,000 G for twenty minutes. 
 
     
     
       9. The method of  claim 1 , wherein the step of analyzing comprises:
 analyzing the phenolic flavonoid matter using LC-MMS, W if a flavonoid material content is at least 10 mg/g of p-coumaric acid, ferulic acid, caffeic acid phenethyl ester value, the process step determining dry matter occurs. 
 
     
     
       10. The method of  claim 1 , wherein the step of analyzing comprises:
 determining if an amount of dry matter is 20 to 30% by weight for product containing ethyl alcohol as solvent and if the amount of the dry matter is 10 to 15% by weight for product containing propylene glycol or glycerol as the solvent and if the extract of the solvent and the dry matter has a difference of 5 to 15% by weight for product containing at least one of the olive oil, the almond oil, the linseed oil, the fish oil, the pomegranate juice, the orange juice, the beetroot juice, and the grape juice, the step of producing the final product occurs. 
 
     
     
       11. The method of  claim 1 , wherein the step of processing the final product comprises:
 adding the extract to a liquid food. 
 
     
     
       12. The method of  claim 1 , wherein the step of processing the final product comprises:
 freeze-drying the extract in powder form.