Methods for detecting lacosamide by mass spectrometry

Abstract

Provided are methods for determining the amount of lacosamide in a sample using mass spectrometry. The methods generally involve ionizing lacosamide in a sample and detecting and quantifying the amount of the ion to determine the amount of lacosamide in the sample.

That which is claimed is: 
     
       1. A method for determining the amount of lacosamide in a sample, said method comprising:
 a. subjecting the sample to protein precipitation; 
 b. purifying lacosamide from said sample by high performance liquid chromatography (HPLC); 
 c. ionizing lacosamide and an internal standard by electrospray ionization (ESI) to produce at least one lacosamide ion and at least one internal standard ion detectable by mass spectrometry, wherein the ions are fragmented with a collision energy of 35V; 
 d. determining an amount of said at least one lacosamide ion and an amount of said at least one internal standard ion by mass spectrometry; and 
 e. comparing the amount of said at least one lacosamide ion and the amount of said at least one internal standard ion to determine the amount of lacosamide in the sample. 
 
     
     
       2. The method of  claim 1 , wherein said mass spectrometry is tandem mass spectrometry. 
     
     
       3. The method of  claim 1 , wherein said sample is a body fluid. 
     
     
       4. The method of  claim 1 , wherein said sample is plasma or serum. 
     
     
       5. The method of  claim 1 , wherein said ionizing comprises generating a lacosamide precursor ion with a mass/charge ratio of 251.0±0.5, and generating one or more lacosamide fragment ions selected from the group consisting of ions with a mass/charge ratio of 91.0±0.5 and 108.0±0.5. 
     
     
       6. The method of  claim 1 , wherein said internal standard is lacosamide-D3. 
     
     
       7. The method of  claim 1 , wherein said ionizing comprises generating an internal standard precursor ion with a mass/charge ratio of 254.0±0.5, and generating an internal standard fragment ion with a mass/charge ratio of 91.0±0.5. 
     
     
       8. The method of  claim 1 , wherein said ionizing is conducted in positive ion mode. 
     
     
       9. A method for determining the amount of lacosamide in a sample, said method comprising:
 (i) subjecting the sample to protein precipitation; 
 (ii) purifying lacosamide from said sample by high performance liquid chromatography (HPLC); 
 (iii) ionizing lacosamide and an internal standard by electrospray ionization (ESI) to produce at least one lacosamide ion and at least one internal standard ion detectable by mass spectrometry, wherein the ions are fragmented with a collision energy of 35V; 
 (iv) determining an amount of said at least one lacosamide ion and an amount of said at least one internal standard ion by mass spectrometry; and 
 (v) comparing the amount of said ions of (iv) determined by mass spectrometry to determine the amount of lacosamide in the sample. 
 
     
     
       10. The method of  claim 9 , wherein said mass spectrometry is tandem mass spectrometry. 
     
     
       11. The method of  claim 9 , wherein said sample is a body fluid. 
     
     
       12. The method of  claim 9 , wherein said sample is plasma or serum. 
     
     
       13. The method of  claim 9 , wherein said ionizing comprises generating a lacosamide precursor ion with a mass/charge ratio of 251.0±0.5, and generating one or more lacosamide fragment ions selected from the group consisting of ions with a mass/charge ratio of 91.0±0.5 and 108.0±0.5. 
     
     
       14. The method of  claim 9 , wherein said internal standard is lacosamide-D3. 
     
     
       15. The method of  claim 9 , wherein said ionizing comprises generating an internal standard precursor ion with a mass/charge ratio of 254.0±0.5, and generating an internal standard fragment ion with a mass/charge ratio of 91.0±0.5. 
     
     
       16. The method of  claim 9 , wherein said ionizing is conducted in positive ion mode.