Methods of selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays

Abstract

The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization.

What is claimed is: 
     
       1. A method for detaching cultured cells comprising
 irradiating cells cultured on a cell culture container with near-infrared radiation to detach the cells from the cell culture container, 
 wherein the cell culture container comprises a cell culture region in which a polymer or copolymer film of at least one monomer selected from the group consisting of compounds of Formulas 1a to 1j having an absorbance in a near-infrared region is formed: 
 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
     
     
       2. The method for detaching cultured cells according to  claim 1 , wherein the cell culture region is formed of any one of polycarbonate, polypropylene, polyethylene, polystyrene, polyurethane, polyethylene terephthalate, polyester, polyimide, polyethylene glycol, polydimethylsiloxane, and a copolymer or composite thereof; Nylon; paper; cotton; and glass. 
     
     
       3. The method for detaching cultured cells according to  claim 1 , wherein the polymer or copolymer has a weight average molecular weight of 1,000 to 1,000,000 Da. 
     
     
       4. The method for detaching cultured cells according to  claim 1 , wherein the polymer or copolymer film has a thickness of 10 nm to 1 mm. 
     
     
       5. The method for detaching cultured cells according to  claim 1 , wherein the cells include adult stem cells obtained from breasts, bone marrow, cord blood, blood, liver, skin, gastrointestine, placenta, or womb.