Composition of natural vitamin C and fish scale collagen peptide and preparation method thereof

Abstract

Disclosed is a composition of natural vitamin C and a fish scale collagen peptide, comprising the following components: natural vitamin C and a fish scale collagen peptide. Also disclosed is a method for preparing the above-mentioned composition.

The invention claimed is: 
     
       1. A method of preparing the composition of natural vitamin C and a fish scale collagen peptide comprising following steps: (1) adding ultrapure water into a formula amount of the fish scale collagen peptide to prepare an aqueous solution; (2) adding a pH regulator into the aqueous solution of the fish scale collagen peptide to adjust a pH value to 5-7; and adding, under a negative pressure condition, a formula amount of the natural vitamin C to prepare a mixed solution of the natural vitamin C and the fish scale collagen peptide; (3) concentrating the mixed solution, obtained in the step (2), under a negative pressure to give a concentrate of ¼-½ of an original volume; and (4) cold drying, freeze drying or spray drying the concentrate under a negative pressure condition, to give the composition of natural vitamin C and the fish scale collagen peptide. 
     
     
       2. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein in the step (1), mass of the ultrapure water is 5-7 times the total mass of the natural vitamin C and the fish scale collagen peptide. 
     
     
       3. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein the pH regulator used in the step (2) for regulating a pH value is citric acid or malic acid. 
     
     
       4. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein in the step (2) and the step (3), temperatures are controlled at 30-55° C. during processes of mixing and concentration. 
     
     
       5. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein in the step (4), a temperature for spray drying is between 100 and 140° C.; a temperature during the process of cold drying is controlled at 40-52° C.; and a temperature during the process of freeze drying is controlled at −20 to −10° C. 
     
     
       6. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein a range for the negative pressures (P) in the steps (2), (3) and (4) is: 0.01 MPa≤P<0.1 MPa. 
     
     
       7. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein the natural vitamin C is extracted from Acerola cherry. 
     
     
       8. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 1 , wherein a method of preparing the fish scale collagen peptide comprises following steps of: a. selecting fish scales as a raw material, and immersing the same in edible vinegar for softening under a negative pressure; b. cleaning, drying and sterilizing the softened fish scales, and adding water and an enzyme successively for enzymatic hydrolysis, wherein pressure is controlled as being negative, during a process of the enzymatic hydrolysis; c. adding activated carbon for decoloration and deodorization under a negative pressure, and filtering out insoluble substances, to give an enzymatic-hydrolyzed fish scale collagen peptide solution; and d. concentrating, under a negative pressure, the fish scale collagen peptide solution to 30-50% of the volume thereof, and obtaining the fish scale collagen peptide by falling film evaporation and drying. 
     
     
       9. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 8 , wherein a range for the negative pressures (P) in the steps a, b, c and d is: 0.01 MPa≤P<0.1 MPa. 
     
     
       10. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 8 , wherein in the step a, temperature of softening is 70-90° C., and duration of softening is 30-50 min. 
     
     
       11. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 8 , wherein in the step b, a weight of water added is 5-20 times that of the fish scales, a weight of the enzyme added is 1%-4% of that of the fish scales, and the enzymatic hydrolysis is performed at a temperature controlled at 30-50° C., for 2-6 h. 
     
     
       12. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 8 , wherein the enzyme is selected from the group consisting of neutral proteases and alkali proteases. 
     
     
       13. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 8 , wherein in the step c, a proportion of the added activated carbon to a weight of the fish scales is 1%-4%, and the decoloration and deodorization are performed at a temperature controlled at 20-30° C., for 30-40 min. 
     
     
       14. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 8 , wherein, in the step d, a temperature is controlled at 60-70° C. during a process of the concentrating, and a temperature is controlled at 100-140° C. during a process of the drying. 
     
     
       15. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 2 , wherein a range for the negative pressures (P) in the steps (2), (3) and (4) is: 0.01 MPa≤P<0.1 MPa. 
     
     
       16. The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to  claim 3 , wherein a range for the negative pressures (P) in the steps (2), (3) and (4) is: 0.01 MPa≤P<0.1 MPa.