USH2181HExpiredUtility
Human tPA production using vectors coding for DHFR protein
Est. expiryJan 19, 2003(expired)· nominal 20-yr term from priority
Inventors:LEVINSON ARTHUR DPENNICA DIANEKOHR WILLIAM JVEHAR GORDON AGOEDDEL DAVID VYELVERTON ELIZABETH MSIMONSEN CHRISTIAN C
C12N 2840/20C12Y 304/21069C12N 15/85C12N 9/6459C12N 9/0028C12N 2800/108
31
PatentIndex Score
1
Cited by
71
References
2
Claims
Abstract
A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
Claims
exact text as granted — not AI-modified1. A method of producing human tissue plasminogen activator, which comprises cultivating a human tissue plasminogen activator-producing recombinant Chinese hamster ovary cell line in a nutrient culture medium which is substantially free of serum and which is substantially free of plasmin and plasminogen.
2. A method of producing human tissue plasminogen activator, which comprises cultivating a DHFR deficient Chinese hamster ovary cell line containing DNA operatively encoding DHFR and human tissue plasminogen activator in a nutrient culture medium which is substantially free of serum and which is substantially free of plasmin and plasminogen.Cited by (0)
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