Power device of a micro channel for external circulation of a bioreactor

Abstract

The present disclosure provides a power device of a micro channel for external circulation of a bioreactor. The power device of the micro channel may be disposed outside the bioreactor and in fluid connection with the bioreactor, the power device of the micro channel may be of a shape of a box body. The power device of the micro channel may comprise: a stacked layer disposed in the box body, including a first shell plate, a second shell plate, and a sealing film sandwiched between the first shell plate and the second shell plate; and a liquid buffer device including a first liquid cavity and a second liquid cavity disposed in the box body, the first liquid cavity and the second liquid cavity may be fixed to an outer end surface of the stacked layer. The power device of the micro channel may be of a box shape, thereby reducing the volume and production cost thereof.

We claim: 
     
         1 . A power device of a micro channel for external circulation of a bioreactor, the power device of the micro channel being disposed outside the bioreactor and in fluid connection with the bioreactor, the power device of the micro channel being of a shape of a box body, the power device of the micro channel comprising:
 a stacked layer disposed in the box body, including a first shell plate, a second shell plate, and a sealing film sandwiched between the first shell plate and the second shell plate; and   a liquid buffer device including a first liquid cavity and a second liquid cavity disposed in the box body, the first liquid cavity and the second liquid cavity being fixed to an outer end surface of the stacked layer; wherein
 a first liquid channel, a second liquid channel, a third liquid channel, and a fourth liquid channel are recessed from an inner surface of the first shell plate; 
 a top of the liquid channel, a top of the second liquid channel, a top of the third liquid channel, and a top of the fourth liquid channel are covered and sealed by the sealing film; 
 the first liquid cavity and the second liquid cavity are in fluid connection with a functional device located downstream of the power device of the micro channel, respectively, via the first liquid channel and the second liquid channel; 
 the first liquid cavity and the second liquid cavity are in fluid connection with the bioreactor, respectively, via the third liquid channel and the fourth liquid channel; 
 the first liquid channel, the second liquid channel, the third liquid channel, and the fourth liquid channel are equipped with a first valve, a second valve, a third valve, and a fourth valve, respectively; 
 each of the first valve, the second valve, the third valve, and the fourth valve includes a valve body cavity and a spool cavity that are in fluid communication with each other; and 
 the valve body cavities are recessed from an inner surface of the first shell plate, and tops of the valve body cavities are covered and sealed by the sealing film. 
   
     
     
         2 . The power device of the micro channel of  claim 1 , wherein the power device of the micro channel further comprises a power device fixed to the box body, and the first liquid cavity and the second liquid cavity are in gas communication with the power device. 
     
     
         3 . The power device of the micro channel of  claim 2 , wherein the power device includes a syringe pump or an air cylinder. 
     
     
         4 . The power device of the micro channel of  claim 1 , wherein a volume of each of the first liquid cavity and the second liquid cavity is 0.01-0.8 bioreactor culture volume. 
     
     
         5 . The power device of the micro channel of  claim 1 , wherein a pressure sensor is installed in at least one of the first liquid channel and the second liquid channel to monitor a clogging state of the downstream functional device. 
     
     
         6 . The power device of the micro channel of  claim 1 , wherein at least one of the first liquid channel, the second liquid channel, the third liquid channel, or the fourth liquid channel is configured on the first shell plate through at least one of machining, laser etching, or soft plastic injection molding. 
     
     
         7 . The power device of the micro channel of  claim 1 , wherein a cross section perpendicular to the first shell plate of at least one of the first liquid channel, the second liquid channel, the third liquid channel, or the fourth liquid channel is semicircular or rectangular, and a diameter or a side length of the at least one of the first liquid channel, the second liquid channel, the third liquid channel, or the fourth liquid channel is in a range from 0.01 mm to 2 mm. 
     
     
         8 . The power device of the micro channel of  claim 7 , wherein the diameter or the side length of the at least one of the first liquid channel, the second liquid channel, the third liquid channel, or the fourth liquid channel is in a range from 0.1 mm to 1 mm. 
     
     
         9 . The power device of the micro channel of  claim 1 , wherein the valve body cavities of the first valve, the second valve, the third valve and the fourth valve are located in the first liquid channel, the second liquid channel, the third liquid channel, and the fourth liquid channel, respectively. 
     
     
         10 . The power device of the micro channel of  claim 9 , wherein a cross section of the valve body cavities perpendicular to the first shell plate is larger than a cross section of a corresponding liquid channel. 
     
     
         11 . The power device of the micro channel of  claim 9 , wherein a cross section of the valve body cavities perpendicular to the first shell plate is circular, elliptical, square, or rectangular. 
     
     
         12 . The power device of the micro channel of  claim 9 , wherein the valve body cavities are configured on the first shell plate through at least one of machining, laser etching, or soft plastic injection molding. 
     
     
         13 . The power device of the micro channel of  claim 9 , wherein the spool cavities are configured as through cavities provided on the second shell plate, and positions of the spool cavities correspond to positions of the valve body cavities. 
     
     
         14 . The power device of the micro channel of  claim 9 , further comprises an additional power device fixed to the box body, and the spool cavities are in fluid communication with the additional power device. 
     
     
         15 . The power device of the micro channel of  claim 14 , wherein the additional power device is configured to push a gas into at least one of the spool cavities to generate a positive pressure in the at least one of the spool cavities, so that the sealing film seals the at least one of the valve body cavities to close a corresponding liquid channel, and is further configured to suction the gas out from the at least one of the spool cavities to generate a negative pressure in the at least one of the spool cavities, so that the sealing film releases the at least one of the valve body cavities to open the corresponding liquid channel. 
     
     
         16 . The power device of the micro channel of  claim 14 , wherein the additional power device is configured to push a spool into at least one of the valve body cavities, so that the sealing film seals the at least one of the valve body cavities to close a corresponding liquid channel, and is further configured to push the spool to leave the at least one of the valve body cavities, so that the sealing film releases the at least one of the valve body cavities to open the corresponding liquid channel. 
     
     
         17 . The power device of the micro channel of  claim 1 , wherein the downstream functional device includes at least one of a cell filtration device, a gas exchange device, or a dialysis device. 
     
     
         18 . The power device of the micro channel of  claim 17 , wherein the cell filtration device includes a hollow fiber column. 
     
     
         19 . The power device of the micro channel of  claim 1 , wherein the first liquid cavity and the second liquid cavity are configured such that
 when the third valve is opened and the first valve is closed, a gas is suctioned out from the first liquid cavity, and the liquid in the bioreactor is suctioned into the first liquid cavity through the third liquid channel, the fourth valve is closed, the second valve is opened, and the gas is pushed into the second liquid cavity, the liquid in the second liquid cavity leaves the second liquid cavity and is pushed to the downstream functional device through the second liquid channel.   
     
     
         20 . The power device of the micro channel of  claim 1 , wherein the first liquid cavity and the second liquid cavity are configured such that
 when the third valve is closed and the first valve is opened, a gas is pushed into the first liquid cavity, and the liquid in the first liquid cavity leaves the first liquid cavity and is pushed into the downstream functional device through the first liquid channel, the fourth valve is opened, the second valve is closed, the gas is suctioned out from the second liquid cavity, and the liquid in the bioreactor is suctioned into the second liquid cavity through the fourth liquid channel.