Microcapsule Material Capable of Reducing Pollution Containing Polycyclic Aromatic Hydrocarbon, and Preparation Method and Application Thereof

Abstract

The present invention discloses a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons, and a preparation method and application thereof. The preparation method includes the following steps: (1) culturing Mycobacterium gilvum CP 13 in a bacteria culture liquid to obtain a bacterial broth, wherein the Mycobacterium gilvum CP 13 was deposited in China General Microbiological Culture Collection Center (CGMCC) on Jul. 22, 2013 with a CGMCC number of CGMCC No. 7963; and (2) applying a calcium alginate and chitosan to encapsulate the bacterial broth in a microcapsule through layer-by-layer self-assembly to produce a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons. The present invention produces a microcapsule material with the microorganic activity through layer-by-layer self-assembly, which has superior adaptability to the environment and good ability to reduce pollution containing polycyclic aromatic hydrocarbons. The microcapsule material of the present invention can be used in bioremediation of industrial wastewater containing polycyclic aromatic hydrocarbons and contaminated soil containing polycyclic aromatic hydrocarbons.

What is claimed is: 
     
         1 . A microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons prepared by the method comprising:
 (1) culturing  Mycobacterium gilvum  CP 13 in a bacteria culture liquid to obtain a bacterial broth; and   (2) encapsulating the bacterial broth in the microcapsules by applying a calcium alginate and chitosan to the bacterial broth through layer-by-layer self-assembly to produce a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons.   
     
     
         2 . A method of degrading polycyclic aromatic hydrocarbons present in at least one of water or soil, wherein the method comprises applying the microcapsule material of  claim 1  to the water and/or soil. 
     
     
         3 . The method according to  claim 2 , wherein the polycyclic aromatic hydrocarbons are pyrene. 
     
     
         4 . The method according to  claim 2 , wherein the microcapsule material is added to water in an amount of 0.1 g of the microcapsule material per 20 ml of water. 
     
     
         5 . The method according to  claim 2 , wherein the microcapsule material is added to soil in an amount of 2.0 g of the microcapsule material per 400 g of soil. 
     
     
         6 . The method according to  claim 2 , wherein the microcapsule material is added to water in an amount of 0.1 g of the microcapsule material per 20 ml of water and added to soil in an amount of 2.0 g of the microcapsule material per 400 g of soil. 
     
     
         7 . The microcapsule material of  claim 1 , wherein the bacteria culture liquid is nutrient broth medium, and wherein the bacteria culture liquid further comprises beef extract 3 g/L, peptone 10 g/L, NaCl 5 g/L, agar 20 g/L, and has a pH in the range of 7.0 to 7.2. 
     
     
         8 . The microcapsule material of  claim 1 , wherein the step (2) of the method further comprises:
 adding chitosan into the bacterial broth so that the chitosan is deposited on a surface of bacteria,   adding the calcium alginate to the bacterial broth so that the calcium alginate is deposited on a surface of chitosan; and   repeating these steps 2-3 times, thereby encapsulating the bacterial broth in the microcapsules.   
     
     
         9 . The microcapsule material of  claim 8 , wherein the concentrations of chitosan and the calcium alginate are each in a range of 0.1-2.0 g/L.