Enzyme electrode

Abstract

The present invention provides an enzyme electrode composed of a carbon particle on which glucose dehydrogenase (GDH) with flavine adenine dinucleotide (FAD) as a coenzyme is supported and an electrode layer contacting the carbon particle, wherein the carbon particle and/or the electrode layer are/is composed of the carbon particles with a particle diameter of not more than 100 nm and a specific surface area of at least 200 m 2 /g.

What is claimed is: 
     
         1 . An enzyme electrode comprising carbon particles carrying glucose dehydrogenase (GDH) with flavine adenine dinucleotide (FAD) as a coenzyme; and
 an electrode layer contacting said carbon particles, wherein the carbon particle is Ketchen black™ and the electrode layer is composed of metal.   
     
     
         2 . The enzyme electrode according to  claim 1 , wherein the glucose dehydrogenase (GDH) is an oxidoreductase catalytic subunit or a complex of an oxidoreductase catalytic subunit and an electron transfer subunit. 
     
     
         3 . The enzyme electrode according to  claim 1 , wherein the carbon particles are composed of carbon particles with a particle diameter of not more than 100 nm and a specific surface area of at least 200 m 2 /g. 
     
     
         4 . The enzyme electrode according to  claim 1 , wherein the enzyme electrode does not require an electron mediator. 
     
     
         5 . The enzyme electrode according to  claim 1 , wherein said metal is gold or stainless. 
     
     
         6 . The enzyme electrode according to  claim 1 , wherein the electrode layer is composed of metal wire. 
     
     
         7 . A glucose sensor comprising the enzyme electrode according to  claim 1 . 
     
     
         8 . A method for producing an enzyme electrode, comprising coating a surface of an electrode layer composed of metal with an ink material and then drying, wherein said ink material comprises glucose dehydrogenase (GDH) with flavine adenine dinucleotide (FAD) as a coenzyme; and carbon particles, wherein the carbon particle is Ketchen black™. 
     
     
         9 . The method according to  claim 8 , further comprising adding a solid polyelectrolyte dropwise and then drying, after the step of coating a surface of an electrode layer with the ink material or after the step of drying. 
     
     
         10 . The method according to  claim 8 , wherein the ink material further comprises the solid polyelectrolyte. 
     
     
         11 . The method according to  claim 8 , wherein the solid polyelectrolyte is Nafion™. 
     
     
         12 . The method according to  claim 8 , wherein the glucose dehydrogenase (GDH) is an oxidoreductase catalytic subunit or a complex of an oxidoreductase catalytic subunit and an electron transfer subunit. 
     
     
         13 . The method according to  claim 8 , wherein the carbon particles are composed of carbon particles with a particle diameter of not more than 100 nm and a specific surface area of at least 200 m 2 /g. 
     
     
         14 . The method according to  claim 8 , wherein the enzyme electrode does not require an electron mediator. 
     
     
         15 . An enzyme fuel cell comprising the enzyme electrode according to  claim 1 . 
     
     
         16 . The enzyme fuel cell according to  claim 15 , wherein the enzyme electrode is used as the anode.