Large stoke shift nir dyes

Abstract

A compound of the following formula: wherein R 1 is SO 3 H; R 2 is chosen from carboxylic acid group and SO 3 H; and R 3 is chosen from Cl, carboxylic acid group, amino, amino-carboxylic acid group, amido group, amino-amido group, and methods of use related to imaging.

1 . A compound of the following formula: 
       
         
           
           
               
               
           
         
         wherein R 1  is SO 3 H; R 2  is chosen from carboxylic acid group and SO 3 H; and R 3  is chosen from: 
       
       
         
           
           
               
               
           
         
       
     
     
         2 . The compound of  claim 1 , wherein R 1  and R 2  are SO 3 H. 
     
     
         3 - 8 . (canceled) 
     
     
         9 . The compound of  claim 1 , wherein R 3  is 
       
         
           
           
               
               
           
         
       
     
     
         10 . The compound of  claim 9 , wherein R 3  is 
       
         
           
           
               
               
           
         
       
     
     
         11 . The compound of  claim 1 , of the following formula: 
       
         
           
           
               
               
           
         
       
     
     
         12 - 13 . (canceled) 
     
     
         14 . A conjugate comprising a biological molecule and a compound chosen from: 
       
         
           
           
               
               
           
         
       
       or 
       
         
           
           
               
               
           
         
       
       or a conjugable form thereof. 
     
     
         15 . The conjugate of  claim 14 , wherein the biological component is chosen from antibodies, proteins, peptides, enzyme substrates, hormones, lymphokines, metabolites, receptors, antigens, haptens, lectins, toxins, carbohydrates, sugars, oligosaccharides, polysaccharides, nucleotides, derivatised nucleotides, nucleic acids, deoxynucleic acids, derivatised nucleic acids, derivatised deoxynucleic acids, DNA fragments, RNA fragments, derivatised DNA fragments, derivatised RNA fragments and drugs. 
     
     
         16 . A method of imaging a sample of cells, comprising:
 providing a compound selected from:   
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         forming a conjugate with said compound and a biological molecule; 
         introducing said conjugate to a sample of cells; 
         exciting said conjugate; 
         detecting said conjugate. 
       
     
     
         17 . The method of  claim 16 , wherein said biological molecule is chosen from antibodies, proteins, peptides, enzyme substrates, hormones, lymphokines, metabolites, receptors, antigens, haptens, lectins, toxins, carbohydrates, sugars, oligosaccharides, polysaccharides, nucleotides, derivatised nucleotides, nucleic acids, deoxynucleic acids, derivatised nucleic acids, derivatised deoxynucleic acids, DNA fragments, RNA fragments, derivatised DNA fragments, derivatised RNA fragments and drugs. 
     
     
         18 . The method of  claim 16 , wherein said compound is of the following formula: 
       
         
           
           
               
               
           
         
       
     
     
         19 . The method of  claim 16 , wherein said compound is of the following formula: 
       
         
           
           
               
               
           
         
       
     
     
         20 . A compound of the following formula: 
       
         
           
           
               
               
           
         
         wherein R 4  is chosen from a carboxylic acid group, amido group, amino-amido group. 
       
     
     
         21 . The compound of  claim 20 , wherein R 4  is chosen from: