Fluorescent spectrum correcting method and fluorescent spectrum measuring device

Abstract

A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.

1 . A fluorescent spectrum correcting method comprising:
 comparing a fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with a reference spectrum to separate the fluorescent spectrum into a fluorescent spectrum for each pigment,   wherein previously measured spectrum data is used as the reference spectrum.   
     
     
         2 . The fluorescent spectrum correcting method according to  claim 1 , wherein in the reference spectrum, an error from a single stain sample is equal to or less than 8%. 
     
     
         3 . The fluorescent spectrum correcting method according to  claim 2 , wherein a measurement date, potential of a detector, a type of coupled antibody, and any spectrum data of different types of cells when the micro-particles are cells are used as the reference spectrum. 
     
     
         4 . The fluorescent spectrum correcting method according to  claim 3 , wherein when the micro-particles are cells, the fluorescent spectrum data measured using cells is used as the reference spectrum. 
     
     
         5 . A fluorescent spectrum measuring device comprising:
 a detection unit that simultaneously detects fluorescent light emitted from micro-particles in an arbitrary wavelength region;   an analysis unit that separates the data detected by the detection unit into a fluorescent spectrum for each pigment; and   a memory unit that stores the fluorescent spectrum data separated by the analysis unit,   wherein the analysis unit uses the previously measured fluorescent spectrum data stored in the memory unit as a reference spectrum to perform separation of the fluorescent spectrum.   
     
     
         6 . The fluorescent spectrum measuring device according to  claim 5 , wherein the detection unit is provided with a multi-channel photo-multiplier tube.