Detection of influenza virus

Abstract

The present application describes methods for detecting influenza A and/or influenza B and/or distinguishing between pathogenic and seasonal influenza A subtypes. Many of these preferred formats employ pan-specific antibodies (i.e., that react with all or at least multiple strains within an influenza type) to detect presence of influenza A and/or influenza B and PDZ domains in combination with panspecific antibodies to influenza A to distinguish pathogenic and seasonal influenza A subtypes.

1 - 38 . (canceled) 
     
     
         39 . A polypeptide comprising multiple copies of a PDZ domain from PSD-95. 
     
     
         40 . The polypeptide of  claim 39 , wherein the PDZ domain is PDZ domain 2 from PSD-95. 
     
     
         41 . The polypeptide of  claim 40 , comprising three copies of PDZ domain 2 from PSD-95. 
     
     
         42 . The polypeptide of  claim 41 , comprising three tandem copies of PSD95 domain 2 interspersed by segments of PSD95 flanking its PDZ domains. 
     
     
         43 . The polypeptide of  claim 42 , in which two of the copies of PSD95 domain 2 replace natural PDZ domains 1 and 3 of the PSD95 polypeptide. 
     
     
         44 . The polypeptide of  claim 39 , having a sequence shown in  FIG. 14A  provided the maltose binding protein moiety may or may not be present. 
     
     
         45 . A polypeptide comprising multiple copies of INADL domain 8. 
     
     
         46 . A kit for diagnosis of influenza virus comprising the polypeptide of  claim 39 . 
     
     
         47 . A kit for diagnosis of influenza virus comprising the polypeptide of  claim 41 . 
     
     
         48 . A kit for diagnosis of influenza virus comprising the polypeptide of  claim 42 . 
     
     
         49 . A kit for diagnosis of influenza virus comprising the polypeptide of  claim 43 . 
     
     
         50 . The kit of  claim 46 , further comprising a second binding agent that can specifically bind to non-structural protein 1 (NS1) of influenza virus type A or B. 
     
     
         51 . The kit of  claim 50 , wherein the polypeptide comprises three tandem copies of PSD95 domain 2 interspersed by segments of PSD95 flanking its PDZ domains, and the second binding agent comprises multiple copies of INADL domain 8. 
     
     
         52 . The kit of  claim 46 , comprising an array of binding agents that can specifically bind to non-structural protein 1 (NS1) of influenza virus type A or B. 
     
     
         53 . The kit of  claim 46 , wherein the polypeptide and/or the second binding agent is attached to a solid support. 
     
     
         54 . The kit of  claim 46 , wherein the solid support is part of a lateral flow assay device. 
     
     
         55 . A polypeptide comprising multiple copies of a PDZ domain from PSD-95 or INADL, attached to a label that can be visually detected. 
     
     
         56 . The polypeptide of  claim 55 , wherein the label is a colorimetric label. 
     
     
         57 . The polypeptide of  claim 39 , wherein the label comprises gold or colored latex particles. 
     
     
         58 . A kit for the diagnosis of an influenza virus infection, comprising the polypeptide of  claim 55  attached to a solid support that is part of a lateral flow device.