Novel agents for replacement of NAD+/NADH system in enzymatic reactions

Abstract

Novel agents acting as co-factors for replacement of NAD(P) + /NAD(P)H co-enzyme systems in enzymatic oxido-reductive reactions. Agents mimicking the action of NAD(P) + /NAD(P)H system in enzymatic oxidation/reduction of substrates into reduced or oxidized products. A method for selection and preparation of the mimicking agents for replacement of NAD(P) + /NAD(P)H system and a device comprising co-factors for replacement of NAD(P) + /NAD(P)H system.

1 - 3 . (canceled).  
     
     
         4 . A device for replacement or regeneration of NAD (P) + /NAD(P)H system in oxido-reductive processes comprising 
 a) a polymer matrix;    b) a catalyst precursor;    c) a co-factor; and    d) an enzyme.    
     
     
         5 . The device of  claim 4  wherein the co-factor is NAD + , NADP +  or a biomimic compound of the formula I or II  
       
         
           
           
               
               
           
         
       
       wherein R is —CN, —C(O)NH 2 , —C(O)NHCH 3 , —C(S)NH 2 , —C(O)CH 3  or —C(O) OCH 3 ; 
 wherein R 1  is —(CH 2 (CH 2 O) n  YR 2  or  
                     
 wherein Y is —OPOO—, —OBO 2 —, —OSO 2 —, CH 3 NH—, —(CH 2 ) n NH—, adenine, or imidazole;  
 wherein R 2  is H, CH 3 , —(OCH 2 CH 2 ) n  R 3 , —(NCH 2 CH 2 ) n [−] R 3  or —[=P (OCH 3 ) 2 ] n  R 3 ;  
 wherein X is —OCH 3 , —CF 3 , —O(CH 2 CH 2 O) n  R 3  or —OPOO R 3 ;  
 wherein R 3  is H, or —CH 3 ,  
 wherein n is 1-2000, or a salt thereof.  
 
     
     
         6 . The device of  claim 5  wherein the co-factor is the biomimic used for replacement of NAD(P) + /NAD(P)H system.  
     
     
         7 . The device of  claim 6  wherein the biomimic is in combination with its 1,4 reduced derivative.  
     
     
         8 . The device of  claim 7  wherein the catalyst precursor is rhodium, zinc, nickel, cobalt, iridium or ruthenium comprising complex.  
     
     
         9 . The device of  claim 8  where the catalyst precursor is rhodium comprising complex.  
     
     
         10 . The device of  claim 9  wherein the catalyst precursor is [Cp*Rh(bpy) (H 2 O)] triflate salt.  
     
     
         11 . The device of  claim 8  additionally comprising a reducing agent.  
     
     
         12 . The device of  claim 11  wherein the reducing agent is formate, hydrogen, sodium borohydride, hydroguinone, sodium borohydrate, and electrode or a photon.  
     
     
         13 . The device of claim  14  wherein the enzyme is oxidase or reductase.  
     
     
         14 - 17 . (Canceled)  
     
     
         18 . A catalyst precursor comprising rhodium, iridium, zinc, cobalt, nickel or ruthenium.