Regeneration of both plant tissues and transgenic plant tissues using a new plant hormone, 5-bromoindole-3-acetic acid

Abstract

The present invention describes the use of 5-bromoindole-3-acetic acid (5-B-IAA) as an auxin affecting plant cell growth. The invention relates to the use of 5-B-IAA compositions to affect growth in monocotyledonous as well as in dicotyledonous plants. The invention also describes the use of 5-B-IAA in plant growth affecting compositions for the regeneration of both plant tissues and transgenic plant tissues. Further, the invention provides plant growth affecting compositions comprising 5-B-IAA alone or in a mixture comprising one or more additional plant growth regulators, such as cytokinin, etc.

We claim:  
     
         1 . A method for affecting the growth of a plant locus, plant cell, plant tissue, immature plant seed, or mature plant seed which comprises applying to said plant locus, pant cell, plant tissue, immature plant seed, or mature plant seed, a growth-affecting, effective amount of a composition comprising 5-bromoindole-3-acetic acid, or a salt, an ester or an amide derivative thereof.  
     
     
         2 . The method of  claim 1  wherein said plant is a plant tissue culture.  
     
     
         3 . The method of  claim 1  wherein said plant is a monocotyledon.  
     
     
         4 . The method of  claim 3  wherein said plant is  Oryza sativa.    
     
     
         5 . The method of  claim 1  wherein said plant is a dicotyledon.  
     
     
         6 . The method of  claim 1  wherein said plant is a transgenic plant.  
     
     
         7 . The method of  claim 6  wherein said plant is a monocotyledon.  
     
     
         8 . The method of  claim 7  wherein said plant is  Oryza sativa.    
     
     
         9 . The method of  claim 6  wherein said plant is a dicotyledon.  
     
     
         10 . The method of  claim 9  wherein said plant is  Arabidopsis thaliana.    
     
     
         11 . The method of  claim 9  wherein said plant is  Nicotiana tabacum.    
     
     
         12 . The method of  claim 1  wherein said composition further comprises an additional plant growth regulator present in an effective amount and ratio to said 5-bromoindole-3-acetic acid to increase root formation.  
     
     
         13 . The method of  claim 1  wherein said composition further comprises an additional plant growth regulator present in an effective amount and ratio to said 5-bromoindole-3-acetic acid to increase shoot formation.  
     
     
         14 . A method for the production of embryogenic callus from a plant comprising contacting a plant cell or plant tissue from said plant with a composition comprising 5-bromoindole-3-acetic acid in an effective amount for formation of embryogenic callus.  
     
     
         15 . The method of  claim 14  wherein said composition further comprises at least one additional plant growth regulator.  
     
     
         16 . The method of  claim 14  wherein said plant is transgenic plant.  
     
     
         17 . The method of  claim 14  wherein said plant is a monocotyledon.  
     
     
         18 . The method of  claim 14  wherein said plant is a dicotyledon.  
     
     
         19 . The method of  claim 14  wherein said plant is selected from the group consisting of  Oryza sativa, Arabidopsis thaliana  and  Nicotiana tabacum.    
     
     
         20 . A kit for the production of embryogenic callus from a plant comprising: 
 at least one container; and    a callus formation medium comprising 5-bromoindole-3-acetic acid and at least one additional plant growth regulator, wherein said 5-bromoindole-3-acetic acid is present in an amount and ratio to said additional plant growth regulator effective for formation of an embryogenic callus.