Chimeric adenoviral fiber protein and methods of using same

Abstract

A recombinant adenovirus comprising a chimeric adenoviral fiber protein comprising a non-adenoviral amino acid sequence in place of or in addition to a native fiber amino acid sequence, wherein the chimeric adenoviral fiber protein comprises a trimerization domain and the non-adenoviral amino acid sequence is of a size such that folding of the chimeric adenoviral fiber protein and assembly of a complex comprising the chimeric adenoviral fiber protein and a penton base is not impeded, and an adenoviral transfer vector for the generation of such a recombinant adenovirus are provided.

What is claimed is:  
     
         1 . A recombinant adenovirus comprising a chimeric adenoviral fiber protein comprising a non-adenoviral amino acid sequence in place of or in addition to a native fiber amino acid sequence, wherein the chimeric adenoviral fiber protein comprises a trimerization domain and the non-adenoviral amino acid sequence is of a size such that folding of the chimeric adenoviral fiber protein and assembly of a complex comprising the chimeric adenoviral fiber protein and a penton base is not impeded.  
     
     
         2 . The recombinant adenovirus of  claim 1 , wherein the non-adenoviral amino acid sequence in the chimeric adenoviral fiber protein is a protein binding sequence  
     
     
         3 . The recombinant adenovirus of  claim 2 , wherein the protein binding sequence is selected from the group consisting of a bispecific protein binding sequence and a multispecific protein binding sequence.  
     
     
         4 . The recombinant adenovirus of  claim 2 , wherein the non-adenoviral amino acid sequence is located at the C-terminus of the chimeric fiber protein.  
     
     
         5 . The recombinant adenovirus of  claim 2 , which further comprises a chimeric coat protein comprising a nonnative amino acid sequence that is specific for binding to a protein.  
     
     
         6 . The recombinant adenovirus of  claim 5 , wherein the chimeric coat protein is selected from the group consisting of a chimeric penton base and a chimeric hexon.  
     
     
         7 . The recombinant adenovirus of  claim 1 , wherein a native fiber receptor binding sequence in the chimeric adenoviral fiber protein is inactive.  
     
     
         8 . A viral transfer vector comprising a chimeric adenoviral fiber protein comprising a non-adenoviral amino acid sequence in place of or in addition to a native fiber amino acid sequence, wherein the chimeric adenoviral fiber protein comprises a trimerization domain and the non-adenoviral amino acid sequence is of a size such that folding of the chimeric adenoviral fiber protein and assembly of a complex comprising the chimeric adenoviral fiber protein and a penton base is not impeded.  
     
     
         9 . The viral transfer vector of  claim 8 , wherein the viral transfer vector is an adenoviral transfer vector.  
     
     
         10 . The viral transfer vector of  claim 9 , wherein the non-adenoviral amino acid sequence in the chimeric fiber protein is a protein binding sequence.  
     
     
         11 . The viral transfer vector of  claim 10 , wherein the protein binding sequence is selected from the group consisting of a bispecific protein binding sequence and a multispecific binding sequence.  
     
     
         12 . The viral transfer vector of  claim 10 , wherein the non-adenoviral amino acid sequence is located at the C-terminus of the chimeric fiber protein.  
     
     
         13 . The viral transfer vector of  claim 9 , wherein a native fiber receptor binding sequence in the chimeric adenoviral fiber protein is inactive.